Serova L I, Korchagin V B, Kotova N I, Tomashchik A D
Antibiotiki. 1976 Apr;21(4):316-20.
A known analytical procedure was used for increasing the sensitivity threshold of the reaction of the zone opening on chromatogrammes. The procedure was the following: the carboxylic acid admixtures determined chromatographically were previously extracted from dry samples of the test-compounds into an optimal solvent. The expediency of the procedure was shown on examples of phenylacetic, phenoxyacetic and isoxasolcarboxylic acids present as admixtures in semisynthetic penicillins and raw materials used for their preparation. The threshold sensitivity was increased by 6 to 9 times. The chromatographic separation was performed in a fixed layer of Silica Gel KSK treated with a buffer. The mobile phase was used in the form of a solvent system: n-heptan-glacial acetic acid (95:5).
采用一种已知的分析方法来提高色谱图上区域开放反应的灵敏度阈值。该方法如下：将色谱测定的羧酸杂质预先从测试化合物的干燥样品中萃取到最佳溶剂中。以半合成青霉素及其制备所用原料中作为杂质存在的苯乙酸、苯氧乙酸和异恶唑羧酸为例，证明了该方法的合理性。阈值灵敏度提高了6至9倍。色谱分离在经缓冲液处理的硅胶KSK固定层中进行。流动相采用溶剂体系的形式：正庚烷 - 冰醋酸（95:5）。
