Strom P F, Matulewich V A, Finstein M S
Appl Environ Microbiol. 1976 May;31(5):731-7. doi: 10.1128/aem.31.5.731-737.1976.
Estimates of NH4+-and NO2-oxidizers in samples from four activated sludge plants treating mainly domestic sewage were obtained using a most-probable-number (MPN) technique. Ranges of concentrations per milliliter of each, respectively, were 1,010 to 3,880 and 79 to 145 in settled sewages, 32 to 7,420 and 2 to 1,010 in secondary effluents, and less than 0.1 to 622 and 0.1 to 70 in chlorinated secondary effluents. The results of this field study indicated that nitrifiers were more resistant to chlorination than fecal streptococci, which were also enumerated. In laboratory studies the survivals of these bacterial groups in secondary effluents were determined after exposure to chlorine residuals of up to 2 mg/liter for 0 to 60 min. The nitrifiers proved considerably more resistant than fecal streptococci, with NO2-oxidizers showing greater resistance than NH4+-oxidizers. Below the outfall of one of the plants that discharges heavily chlorinated unnitrified effluent, NH4+-oxidizers amounted to approximately 200 X 10(5) per g of slime scraped from stream-bed rocks. Upstream of the outfall this was approximatley 3 X 10(5)/G.
采用最大可能数（MPN）技术，对四座主要处理生活污水的活性污泥厂的样本中的铵离子氧化菌和亚硝酸根离子氧化菌进行了估算。在沉淀污水中，每毫升各自的浓度范围分别为1010至3880和79至145，在二级出水中为32至7420和2至1010，在氯化二级出水中小于0.1至622和0.1至70。这项现场研究的结果表明，硝化菌比粪链球菌更耐氯化，同时也对粪链球菌进行了计数。在实验室研究中，将这些细菌群体暴露于高达2毫克/升的余氯中0至60分钟后，测定了它们在二级出水中的存活率。结果证明，硝化菌比粪链球菌更具抗性，其中亚硝酸根离子氧化菌比铵离子氧化菌表现出更强的抗性。在一座排放大量氯化未硝化废水的工厂的排水口下游，从河床岩石上刮下的每克黏液中铵离子氧化菌数量约为200×10⁵。在排水口上游，这一数量约为3×10⁵/克。
