Endou H, Kimura K, Sudo J I, Sakai F
Jpn J Exp Med. 1978 Aug;48(4):297-301.
Electrophoresis of 125I-labelled rat albumin from 0.5 to 700 ng was performed with a 5 microliter capillary tube in which 4-40% continuous gradient polyacrylamide gel was prepared. The linear relationship was obtained by using double logarithm plots between protein amounts and densitometric areas of the protein bands stained with Coomassie blue. The radioactivities of the same gels and the protein amounts were also shown to be linear. In the case of rat gamma-globulin, the linear relationship was also obtained. As an applied example of this method, the urinary gamma-globulin excretion showed marked changes caused by the circadian rhythm in rats.
用一根5微升的毛细管对0.5至700纳克的125I标记大鼠白蛋白进行电泳，毛细管中制备了4 - 40%的连续梯度聚丙烯酰胺凝胶。通过使用考马斯亮蓝染色的蛋白质条带的蛋白质含量与光密度面积之间的双对数图获得线性关系。相同凝胶的放射性与蛋白质量也呈线性关系。对于大鼠γ-球蛋白，也得到了线性关系。作为该方法的一个应用实例，大鼠尿γ-球蛋白排泄量显示出由昼夜节律引起的显著变化。
