与母羊泌乳乳腺中结合多核糖体相关的mRNA-蛋白质复合物的分离及特性研究

Isolation and characteristics of mRNPs associated with bound polyribosomes from the ewe lactating mammary gland.

作者信息

Assairi L, Gaye P, Houdebine L M

出版信息

Biochimie. 1976;58(3):325-31. doi: 10.1016/s0300-9084(76)80439-7.

PMID:819040

Abstract

The dissociation of bound polyribosomes from ewe lactating mammary gland, by EDTA or Puromycin-KC1 treatment releases Messenger Ribonucleoprotein particles (mRNP'S) which sediment in sucrose gradients as a single component having a sedimentation coefficient of about 22S. RNAs derived from mRNPs sediment between 9S and 15S and direct the synthesis of milk proteins in reticulocyte cell free systems. The mRNP protein moiety contains two major polypeptides with molecular weights of 52,000 and 72,000 daltons and two minor polypeptides with apparent molecular weights of 58,000 and 60,000 daltons.

摘要

通过用乙二胺四乙酸（EDTA）或嘌呤霉素 - 氯化钾处理，从母羊泌乳乳腺中解离结合的多核糖体，释放出信使核糖核蛋白颗粒（mRNP），这些颗粒在蔗糖梯度中作为单一成分沉降，沉降系数约为22S。源自mRNP的RNA沉降在9S至15S之间，并在无细胞网织红细胞系统中指导乳蛋白的合成。mRNP蛋白部分包含两种主要多肽，分子量分别为52,000和72,000道尔顿，以及两种次要多肽，表观分子量分别为58,000和60,000道尔顿。