从泌乳兔乳腺中分离出的游离多核糖体上脂肪酸合成酶信使核糖核酸的鉴定。
Identification of fatty acid synthetase messenger RNA on free polyribosomes isolated from lactating rabbit mammary gland.
作者信息
Antoniou M, Craig R, Dils R
出版信息
Biochem J. 1981 Dec 1;199(3):789-93. doi: 10.1042/bj1990789.
Abstract
The synthesis of fatty acid synthetase on free polyribosomes from lactating rabbit mammary gland was demonstrated by using polyribosomes run-off techniques and immunochemical identification of products with synthetase antiserum. Several reproducible and discrete immunoprecipitable polypeptides were observed which were within the molecular-weight range of the synthetase subunit (235 000--252 000), as well as several of lower molecular weight.
摘要
通过使用多核糖体溢流技术以及用合成酶抗血清对产物进行免疫化学鉴定，证实了来自泌乳兔乳腺的游离多核糖体上脂肪酸合成酶的合成。观察到了几种可重复且离散的可免疫沉淀多肽，它们的分子量在合成酶亚基的分子量范围内（235000 - 252000），还有几种分子量较低的多肽。
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