[通过红螺菌的分离色素 - 蛋白质复合物重建膜电位形成功能]

[Reconstruction of the function of membrane potential formation by isolated pigment-protein complexes of Rhodospirillum rubrum].

作者信息

Barskiĭ E L, Kondrashin A A, Samuilov V D, Skulachev V P

出版信息

Biokhimiia. 1976 Mar;41(3):513-9.

Abstract

The pigment-protein complexes, containing the photosynthetic reaction centers and chlorophyll antenna, were isolated from non-sulfur purple bacteria Rhodospirillum rubrum, by use of cholate. Absorption spectra and quantum yield of photo-oxidation of bacteriochlorophyll P870 of complexes were similar to those of chromatophores. The procedure of reconstitution of proteoliposomes from phospholipids and complexes was proposed. The proteoliposomes incubated with CoQ6 and TMPD upon illumination are generating the transmembrane electric potential difference (plus inside proteoliposomes). The electric potential difference was registered by three independent methods: by transmembrane electrophoresis of penetrating anions PCB-, by direct measurement with using voltmeter in system "proteoliposome--planar membrane" and electrochromic absorption band shifts of bacteriochlorophyll.

摘要

利用胆酸盐从非硫紫色细菌深红红螺菌中分离出了含有光合反应中心和叶绿素天线的色素 - 蛋白质复合物。复合物中细菌叶绿素P870的吸收光谱和光氧化量子产率与载色体的相似。提出了由磷脂和复合物重构蛋白脂质体的方法。光照下与辅酶Q6和四甲基对苯二胺一起孵育的蛋白脂质体产生跨膜电势差（蛋白脂质体内部为正）。通过三种独立方法记录电势差：穿透性阴离子PCB - 的跨膜电泳、在“蛋白脂质体 - 平面膜”系统中使用电压表直接测量以及细菌叶绿素的电致变色吸收带位移。

相似文献

1

[Reconstruction of the function of membrane potential formation by isolated pigment-protein complexes of Rhodospirillum rubrum].[通过红螺菌的分离色素 - 蛋白质复合物重建膜电位形成功能]

Biokhimiia. 1976 Mar;41(3):513-9.

2

Generation of electric current by chromatophores of Rhodospirillum rubrum and reconstitution of electrogenic function in subchromatophore pigment-protein complexes.深红红螺菌的载色体产生电流及亚载色体色素-蛋白质复合物中电生功能的重建。

Biochim Biophys Acta. 1976 Sep 13;440(3):637-60. doi: 10.1016/0005-2728(76)90048-7.

3

Reconstitution of biological molecular generators of electric current. Bacteriochlorophyll and plant chlorophyll complexes.生物电流分子发生器的重构。细菌叶绿素与植物叶绿素复合体。

J Biol Chem. 1976 Nov 25;251(22):7066-71.

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Biokhimiia. 1977 Nov;42(11):1997-2004.

6

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8

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[Dicyclohexylcarbodiimide as an inhibitor of light- and pyrophosphate-induced formation of membrane potential in chromatophores of purple bacteria].[二环己基碳二亚胺作为紫色细菌载色体中光和焦磷酸诱导的膜电位形成的抑制剂]

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10

[Photoinduced absorption of hydrophobic penetrating anions by Rhodospirillum rubrum non-sulfur purple bacteria].

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