Kondrashin A A, Remennikov V G, Samuilov V D, Skulachev V P
Biokhimiia. 1979 Nov;44(11):2103-6.
The membrane vesicles (proteoliposomes) have been reconstituted from phospholipids and inorganic pyrophosphatase (EC 3.6.1.1) isolated from Rhodospirillum rubrum chromatophores. An addition of inorganic pyrophosphate (PPi) causes a Mg2+-dependent formation of a transmembrane electric potential difference and an uptake of penetrating tetraphenylborate anions by the proteoliposomes. Thus, isolated pyrophosphatase, being incorporated into the phospholipid membrane, functions as a MgPPi-dependent protein generator of the electric current.
膜囊泡（蛋白脂质体）由从红螺菌载色体中分离出的磷脂和无机焦磷酸酶（EC 3.6.1.1）重构而成。添加无机焦磷酸（PPi）会导致蛋白脂质体形成Mg2+依赖性的跨膜电势差，并摄取可穿透的四苯硼酸根阴离子。因此，分离出的焦磷酸酶掺入磷脂膜后，可作为一种依赖MgPPi的电流蛋白发生器发挥作用。
