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1
The differences in the T2 relaxation rates of the protons in the partially-deuteriated and fully protonated sugar residues in a large oligo-DNA ('NMR-window') gives complementary structural information.大型寡聚 DNA（“核磁共振窗口”）中部分氘代和完全质子化糖残基中质子的 T2 弛豫率差异提供了互补的结构信息。
Nucleic Acids Res. 1994 Apr 25;22(8):1404-12. doi: 10.1093/nar/22.8.1404.
2
Deuteriation of sugar protons simplify NMR assignments and structure determination of large oligonucleotide by the 1H-NMR window approach.糖质子的氘代通过¹H-NMR窗口方法简化了大型寡核苷酸的NMR归属和结构测定。
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The identification of the A-type RNA helices in a 55mer RNA by selective incorporation of deuterium-labelled nucleotide residues (Uppsala NMR-window concept).通过选择性掺入氘标记的核苷酸残基（乌普萨拉核磁共振窗口概念）来鉴定55聚体RNA中的A型RNA螺旋。
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Solution structure of GCCAAT recognition motif by 2D NMR, spectral simulation, molecular modeling, and distance geometry calculations.通过二维核磁共振、光谱模拟、分子建模和距离几何计算得到的GCCAAT识别基序的溶液结构。
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1
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Sugar conformation of a stereospecific 2'-R or 2'-S deuterium-labeled DNA decamer studied with proton-proton J coupling constants.利用质子-质子J耦合常数研究立体特异性2'-R或2'-S氘标记DNA十聚体的糖构象。
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3
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6
The use of non-uniform deuterium labelling ['NMR-window'] to study the NMR structure of a 21mer RNA hairpin.使用非均匀氘标记法['核磁共振窗口']研究一个21聚体RNA发夹的核磁共振结构。
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Deuterium labelling in NMR structural analysis of larger proteins.
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Solution studies of staphylococcal nuclease H124L. 2. 1H, 13C, and 15N chemical shift assignments for the unligated enzyme and analysis of chemical shift changes that accompany formation of the nuclease-thymidine 3',5'-bisphosphate-calcium ternary complex.葡萄球菌核酸酶H124L的溶液研究。2. 未结合配体的酶的1H、13C和15N化学位移归属以及核酸酶-胸腺嘧啶3',5'-二磷酸-钙三元复合物形成过程中伴随的化学位移变化分析。
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The octamer motif in immunoglobulin genes: extraction of structural constraints from two-dimensional NMR studies.免疫球蛋白基因中的八聚体基序：从二维核磁共振研究中提取结构限制因素
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The effect of selective deuteration on magnetization transfer in larger proteins.
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Preparation of isotopically labeled ribonucleotides for multidimensional NMR spectroscopy of RNA.用于RNA多维核磁共振光谱分析的同位素标记核糖核苷酸的制备
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Preparation of 13C and 15N labelled RNAs for heteronuclear multi-dimensional NMR studies.用于异核多维核磁共振研究的¹³C和¹⁵N标记RNA的制备。
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大型寡聚 DNA（“核磁共振窗口”）中部分氘代和完全质子化糖残基中质子的 T2 弛豫率差异提供了互补的结构信息。

The differences in the T2 relaxation rates of the protons in the partially-deuteriated and fully protonated sugar residues in a large oligo-DNA ('NMR-window') gives complementary structural information.

作者信息

Agback P, Maltseva T V, Yamakage S I, Nilson F P, Földesi A, Chattopadhyaya J

机构信息

Department of Bioorganic Chemistry, University of Uppsala, Sweden.

出版信息

Nucleic Acids Res. 1994 Apr 25;22(8):1404-12. doi: 10.1093/nar/22.8.1404.

DOI:10.1093/nar/22.8.1404
PMID:8190632
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307998/
Abstract

Selective incorporation of the stereospecifically deuteriated sugar moieties (> 97 atom % 2H enhancements at H2', H2'', H3' and H5'/5'' sites, approximately 85 atom % 2H enhancement at H4' and approximately 20 atom % 2H enhancement at H1') in DNA and RNA by the 'NMR-window' approach has been shown to solve the problem of the resonance overlap [refs. 1, 2 & 3]. Such specific deuterium labelling gives much improved resolution and sensitivity of the residual sugar proton (i.e. H1' or H4') vicinal to the deuteriated centers (ref. 3). The T2 relaxation time of the residual protons also increases considerably in the partially-deuteriated (shown by underline) sugar residues in dinucleotides [d(CpG), d(GpC), d(ApT), d(TpA)], trinucleotide r(A2'p5'A2'p5'A) and 20-mer DNA duplex 5'd(C1G2C3-G4C5G6C7G8A9A10T11T12C13G14C15G16C17G18C19G20)(2) 3'. The protons with shorter T2 can be filtered away using a number of different NMR experiments such as ROESY, MINSY or HAL. The NOE intensity of the cross-peaks in these experiments includes only straight pathway from H1' to aromatic proton (i-i and i-i + 1) without any spin-diffusion. The volumes of these NOE cross-peaks could be measured with high accuracy as their intensity is 3 to 4 times larger than the corresponding peaks in the fully protonated residues in the normal NOESY spectra. The structural informations thus obtainable from the residual protons in the partially-deuteriated part of the duplex and the fully protonated part in the 'NMR window' can indeed complement each other.

摘要

通过“NMR窗口”方法在DNA和RNA中选择性掺入立体特异性氘代糖部分（H2'、H2''、H3'和H5'/5''位点的氘增强>97原子%，H4'的氘增强约85原子%，H1'的氘增强约20原子%）已被证明可解决共振重叠问题[参考文献1、2和3]。这种特定的氘标记大大提高了与氘代中心相邻的残留糖质子（即H1'或H4'）的分辨率和灵敏度（参考文献3）。在二核苷酸[d（CpG）、d（GpC）、d（ApT）、d（TpA）]、三核苷酸r（A2'p5'A2'p5'A）和20聚体DNA双链体5'd（C1G2C3 - G4C5G6C7G8A9A10T11T12C13G14C15G16C17G18C19G20）（2）3'中，部分氘代（下划线表示）糖残基中残留质子的T2弛豫时间也显著增加。可以使用许多不同的NMR实验，如ROESY、MINSY或HAL，滤去T2较短的质子。这些实验中交叉峰的NOE强度仅包括从H1'到芳香族质子的直接路径（i-i和i-i + 1），没有任何自旋扩散。这些NOE交叉峰的体积可以高精度测量，因为它们的强度比正常NOESY光谱中完全质子化残基中的相应峰大3到4倍。因此，从双链体部分氘代部分的残留质子和“NMR窗口”中完全质子化部分可获得的结构信息确实可以相互补充。