Dong Y, Sirotkin A M, Yang Y S, Brown D T, Sittman D B, Skoultchi A I
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461.
Nucleic Acids Res. 1994 Apr 25;22(8):1421-8. doi: 10.1093/nar/22.8.1421.
Mice contain at least seven nonallelic forms of the H1 histones, including the somatic variants H1a-e and less closely related variants H1 degrees and H1t. The mouse H1 degrees and H1c (H1var.1) genes were isolated and characterized previously. We have now isolated, sequenced and studied the expression properties of two additional mouse H1 genes, termed H1var.2 and H1var.3. Extensive amino acid and nucleotide sequence comparisons were made between the two genes and other mammalian H1 histone genes. A high degree of nucleotide sequence identity was seen between the H1var.2, rat H1d and human H1b genes, even well beyond the coding region, indicating that these genes are likely homologues. Unlike the previously characterized mouse H1var.1 gene which produces both nonpolyadenylated and polyadenylated mRNAs, the H1var.2 and H1var.3 genes produce only typical, replication dependent, nonpolyadenylated mRNAs.
小鼠含有至少七种非等位形式的H1组蛋白，包括体细胞变体H1a - e以及关系不太密切的变体H1°和H1t。小鼠H1°和H1c（H1var.1）基因先前已被分离和鉴定。我们现在已经分离、测序并研究了另外两个小鼠H1基因的表达特性，这两个基因被称为H1var.2和H1var.3。对这两个基因与其他哺乳动物H1组蛋白基因进行了广泛的氨基酸和核苷酸序列比较。在H1var.2、大鼠H1d和人类H1b基因之间观察到高度的核苷酸序列同一性，甚至远远超出编码区，表明这些基因可能是同源物。与先前鉴定的产生非聚腺苷酸化和聚腺苷酸化mRNA的小鼠H1var.1基因不同，H1var.2和H1var.3基因仅产生典型的、依赖复制的非聚腺苷酸化mRNA。
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