Cole K D, Kandala J C, Kremer E, Kistler W S
Department of Chemistry, University of South Carolina, Columbia 29208.
Gene. 1990 May 14;89(2):265-9. doi: 10.1016/0378-1119(90)90015-j.
Mammals contain a family of five closely related H1 histone variants (H1a-e) as well as two less closely related forms, H10 and H1t. We have sequenced a rat genomic clone that encodes one of the standard H1 variants. An RNA transcript of the gene was made with bacteriophage SP6 RNA polymerase and translated in a cell-free system. The protein synthesized in vitro was identified as variant H1d by its electrophoretic mobility.
哺乳动物包含一个由五个紧密相关的H1组蛋白变体（H1a - e）以及两个相关性稍弱的形式H10和H1t组成的家族。我们对一个编码标准H1变体之一的大鼠基因组克隆进行了测序。该基因的RNA转录本由噬菌体SP6 RNA聚合酶合成，并在无细胞系统中进行翻译。通过其电泳迁移率，体外合成的蛋白质被鉴定为变体H1d。
