Mitsuno K, Sasa M, Ishihara K, Ishikawa M, Kikuchi H
Department of Neurosurgery, Faculty of Medicine, Kyoto University, Japan.
Physiol Behav. 1994 Apr;55(4):633-8. doi: 10.1016/0031-9384(94)90037-x.
The study was done to determine whether long-term potentiation (LTP) of the potentials occurs at mossy fiber (MF)-CA3 synapses with the advance of learning in unrestrained and unanesthetized rats. The rats were divided into two groups, the test and control groups. The test group was given daily learning tasks in the radial arm maze, whereas the control group was similarly handled without learning tasks. Complete acquisition of learning was observed in the test group on day 5, and the learning was maintained over 3 days. Under freely moving conditions, a significant increase in population spikes (PS) elicited by MF stimulation with the progress in learning was observed in the test group, and the PS potentiation remained stable after day 4. Furthermore, on day 7, when MF stimulation-induced PS in the test group were compared with that in the control group in pentobarbital-anesthetized rats, the responses were comparatively higher in the former. As the training-induced PS potentiation in CA3 occurred with the advance of learning, these findings suggest that LTP in CA3 induced by learning may be related to memory storage.
本研究旨在确定在未束缚、未麻醉的大鼠中，随着学习进程的推进，苔藓纤维（MF）-CA3突触处是否会出现电位的长时程增强（LTP）。大鼠被分为两组，即测试组和对照组。测试组每天在放射状臂迷宫中接受学习任务，而对照组则在不进行学习任务的情况下进行类似处理。测试组在第5天观察到学习完全习得，且学习持续了3天。在自由活动条件下，测试组中随着学习进程，MF刺激诱发的群体峰电位（PS）显著增加，且在第4天后PS增强保持稳定。此外，在第7天，当将戊巴比妥麻醉大鼠中测试组的MF刺激诱发的PS与对照组的进行比较时，前者的反应相对较高。由于CA3中训练诱导的PS增强随着学习进程而发生，这些发现表明学习诱导的CA3中的LTP可能与记忆存储有关。
