Lobb D K, Dorrington J H
Department of Obstetrics and Gynaecology, McMaster University, Hamilton, Ontario, Canada.
Reprod Fertil Dev. 1993;5(5):523-9. doi: 10.1071/rd9930523.
Transforming growth factor-alpha (TGF-alpha), a product of the thecal cells, has potent mitogenic and steroidogenic influences on cells within the ovarian follicle. Whether TGF-alpha continues to be produced in those follicles that go on to ovulate and form a corpus luteum is currently under investigation. In the present study, TGF-alpha was localized in the bovine corpus luteum by means of immunoperoxidase staining using a monoclonal antibody for TGF-alpha that does not cross-react with epidermal growth factor. In corpora lutea from the mid-luteal phase of the cycle TGF-alpha staining was found predominantly in the large luteal cells. Northern blot analysis using a human TGF-alpha cDNA probe hybridized to the 4.5-4.8 kb TGF-alpha transcript in RNA from the corpus luteum. These studies provide new evidence that TGF-alpha, a potent paracrine regulator within the ovarian follicle, continues to be expressed in the corpus luteum.
转化生长因子-α（TGF-α）是卵泡膜细胞的产物，对卵巢卵泡内的细胞具有强大的促有丝分裂和类固醇生成作用。目前正在研究那些继续排卵并形成黄体的卵泡中是否仍会产生TGF-α。在本研究中，通过使用针对TGF-α且不与表皮生长因子交叉反应的单克隆抗体进行免疫过氧化物酶染色，将TGF-α定位在牛黄体中。在周期黄体中期的黄体中，TGF-α染色主要见于大黄体细胞。使用人TGF-α cDNA探针进行的Northern印迹分析显示，该探针与来自黄体的RNA中的4.5 - 4.8 kb TGF-α转录本杂交。这些研究提供了新的证据，表明TGF-α作为卵巢卵泡内一种强大的旁分泌调节因子，在黄体中持续表达。
