Robert A
Laboratoire Central d'Hématologie, Hôpital Saint-Antoine, Paris, France.
Thromb Haemost. 1994 Feb;71(2):220-4.
We describe a test for LA based on the specific APTT behaviour of LA plasmas when the incubation time with the APTT reagent is increased from 1 to 20 min. "1-10 APTT" test was defined as the difference (s) between results of the APTT performed with 1 and the one performed with 10 min incubation. A test value > 11 s (upper normal limit determined on 134 normal plasmas) was considered positive for a LA. The test distinguished all the LA patients studied (n = 40) from patients with factor VIIIc inhibitors, patients receiving heparin or warfarin therapy and also patients with congenital factor deficiencies, except those with prekallikrein and factor XII deficiencies. The test detected LA in warfarin (n = 3) and in heparin (n = 2) LA anticoagulated patients. Among 195 patients referred for LA screening, the test detected LA in 5 patients with normal standard APTT. This simple test, using a single reagent for screening and confirmatory procedures is sensitive and fairly specific for LA when combined with mixing studies. However, since the test was defined using one APTT reagent, the performances of other reagents have to be assessed.
我们描述了一种基于狼疮抗凝物（LA）血浆与活化部分凝血活酶时间（APTT）试剂孵育时间从1分钟增加到20分钟时特定APTT行为的LA检测方法。“1 - 10 APTT”检测定义为1分钟孵育和10分钟孵育时APTT结果之间的差值。检测值>11秒（根据134份正常血浆确定的正常上限）被认为LA检测呈阳性。该检测方法能够区分所有研究的LA患者（n = 40）与VIII因子c抑制剂患者、接受肝素或华法林治疗的患者以及先天性因子缺乏患者，但激肽释放酶原和XII因子缺乏的患者除外。该检测方法在服用华法林（n = 3）和肝素（n = 2）的LA抗凝患者中检测到了LA。在195名因LA筛查而转诊的患者中，该检测方法在5名标准APTT正常的患者中检测到了LA。这种简单的检测方法，在筛查和确认过程中使用单一试剂，与混合试验相结合时，对LA具有敏感性和相当的特异性。然而，由于该检测方法是使用一种APTT试剂定义的，其他试剂的性能必须进行评估。
