Boersma A, Lamblin G, Roussel P, Degand P, Biserte G
C R Acad Hebd Seances Acad Sci D. 1975 Oct 27;281(17):1269-72.
Endo beta-N-acetylglucosaminidase activity was found in a alpha-L-fucosidase preparation purified from human liver homogenates, using glycopeptides from human parotid glycoprotein as a substrate. An oligosaccharide was purified and after reduction N-acetylglucosaminitol was shown by paper chromatography. Thus, the specificity and the significance of this endoglycosidase in glycoprotein catabolism are discussed.
使用人腮腺糖蛋白的糖肽作为底物,在从人肝脏匀浆中纯化得到的α-L-岩藻糖苷酶制剂中发现了内切β-N-乙酰氨基葡萄糖苷酶活性。一种寡糖被纯化出来,经还原后,通过纸层析法显示出N-乙酰葡糖胺醇。因此,本文讨论了这种内切糖苷酶在糖蛋白分解代谢中的特异性和意义。