Czirók E, Herpay M, Milch H
B. Johan National Institute of Hygiene, Budapest, Hungary.
Acta Microbiol Hung. 1993;40(3):217-37.
A multivariate analysis of 3334 Escherichia coli strains originating from different clinical materials revealed that 50.2% of isolates belonged to the most common 12 (O1, O2, O4, O6, O7, O8, O15, O18, O45, O75, O78, O83) out of 133 serogroups. Haemolysin (Hly) production, mannose resistant haemagglutinating activity for human erythrocytes (MRHA) and colicinogenicity (Col) were recorded in 30, 30 and 36%, respectively. Antigens K1 and K5 were present in 11% and 6.6%, respectively. Association were found among certain serotypes and virulence markers (O1, H-, H7, K1, MRHA, Col; O2, H-, Kl, Col; O4, H-, H5, MRHA, Hly; O6, H-, H1, MRHA, Hly; O6, K5, MRHA, Col; O7, H-, H4, K1, MRHA, Col; O18ac, H7, K1, Col; O18ac, H-, K5, MRHA, Hly; O78, H-, Col (V-type); O83, H-, K1, Col). There were associations among clinical specimens, age of patients, nosocomial group of diseases, serogroups and virulence markers, too (cerebrospinal fluid-CSF-O7, O18ac, O45, O83-K1-newborn meningitis; O78-ColV-meningitis, sepsis, inflammations diseases of premature babies; CFS-O6, MRHA, Hly-adult-meningitis, sepsis, urinary tract infection-UTI-, pneumonia, other inflammatory diseases; blood-O2, O4, O6, O18ac, ONT, K5, MRHA, Hly-sepsis, UTI, hepatic diseases; urine-O1, O2, O4, O6, O18ac, O75, virulence markers fall to differ among upper and lower UTI; faeces-O1, O4, O6, O18ac, O78, virulence markers rare). Associations were also found among animal pathogenicity tests, specimens, serogroups and virulence factors: highly virulent group strains (i.e. LD50 below 10(6)) belonged to serogroups O2, O6, O18ac, possessed antigen K1 (less frequently the presence of MRHA, Hly, K5) and originated mainly from CSF. With mouse lung toxicity test correlations of serogroups (O4, O6, O18ac), antigen K5, MRHA, Hly and specimens (blood) were also shown. However, association was found between the lack of virulence factors and phage insensitivity and also between K5 positivity and sensitivity to phages 16, 17, there were no correlations between serogroups and phage patterns. On the basis of the above-described associations one can find correlations among virulence markers, serotype, and nosological group of diseases. Animal pathogenicity tests give additional data in understanding the pathomechanism of diseases. Correlations between phage patterns and serogroups reveal certain epidemiological relatedness and also virulence of strains.
对来自不同临床材料的3334株大肠杆菌进行的多变量分析显示，在133个血清群中，50.2%的分离株属于最常见的12个血清群（O1、O2、O4、O6、O7、O8、O15、O18、O45、O75、O78、O83）。溶血素（Hly）产生、对人红细胞的甘露糖抗性血凝活性（MRHA）和产大肠杆菌素性（Col）的记录率分别为30%、30%和36%。抗原K1和K5的存在率分别为11%和6.6%。在某些血清型与毒力标记之间发现了关联（O1、H-、H7、K1、MRHA、Col；O2、H-、Kl、Col；O4、H-、H5、MRHA、Hly；O6、H-、H1、MRHA、Hly；O6、K5、MRHA、Col；O7、H-、H4、K1、MRHA、Col；O18ac、H7、K1、Col；O18ac、H-、K5、MRHA、Hly；O78、H-、Col（V型）；O83、H-、K1、Col）。临床标本、患者年龄、医院疾病组、血清群和毒力标记之间也存在关联（脑脊液-CSF-O7、O18ac、O45、O83-K1-新生儿脑膜炎；O78-ColV-脑膜炎、败血症、早产儿炎症性疾病；脑脊液-O6、MRHA、Hly-成人-脑膜炎、败血症、尿路感染-UTI-、肺炎、其他炎症性疾病；血液-O2、O4、O6、O18ac、ONT、K5、MRHA、Hly-败血症、UTI、肝脏疾病；尿液-O1、O2、O4、O6、O18ac、O75，上、下UTI的毒力标记无差异；粪便-O1、O4、O6、O18ac、O78，毒力标记罕见）。在动物致病性试验、标本、血清群和毒力因子之间也发现了关联：高毒力组菌株（即半数致死量低于10⁶）属于血清群O2、O6、O18ac，具有抗原K1（较少出现MRHA、Hly、K5），主要源自脑脊液。小鼠肺毒性试验还显示了血清群（O4、O6、O18ac）、抗原K5、MRHA、Hly与标本（血液）之间的相关性。然而，发现毒力因子缺乏与噬菌体不敏感性之间以及K5阳性与对噬菌体16、17的敏感性之间存在关联，血清群与噬菌体型之间无相关性。基于上述关联，可以发现毒力标记、血清型和疾病病种之间存在相关性。动物致病性试验为理解疾病的发病机制提供了额外的数据。噬菌体型与血清群之间的相关性揭示了某些流行病学相关性以及菌株的毒力。
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