Czirók E, Petheö G, Szöllösy E, Milch H, Herpay M, Csík M
National Institute of Hygiene, Budapest, Hungary.
Acta Microbiol Hung. 1987;34(3-4):219-24.
Living suspensions of 89 Escherichia coli strains were tested for adsorption to Al(OH)3 gel in the presence of phosphate ions. On the basis of AC50 (phosphate molarity inhibiting 50% adsorption of the strain examined), E. coli strains could be classified into two main groups. Forty-three strains belonged to group 1 (AC50, 0.01-0.04), and 42 of them fell into serogroups O1, O2, O5, O7, O18ac, O83 or were spontaneously agglutinable. One strain in group 1 was exceptional as it had antigen O4. Of these 43 strains 33 had K antigen K1. Serogroup distribution of 46 group 2 strains (AC50, 0.001-0.009) was O2, O4, O6, O18ac, O75 and O78; 20 out of 46 possessed antigen K5. No correlation existed between H antigens or haemagglutinating capacity and AC50 of the strains. A close correlation was shown between AC50 pattern and the two main pathogenecity groups (i.e. "newborns' meningitis" and "sepsis and organotropic diseases") on one hand and between AC50 pattern and O, K serotypes on the other. The findings indicate that these E. coli strains with identical markers had a clonal connection.
对89株大肠杆菌的活悬液在磷酸根离子存在的情况下吸附到氢氧化铝凝胶上的情况进行了测试。根据AC50（抑制所检测菌株50%吸附的磷酸盐摩尔浓度），大肠杆菌菌株可分为两个主要组。43株属于第1组（AC50为0.01 - 0.04），其中42株属于血清群O1、O2、O5、O7、O18ac、O83或可自发凝集。第1组中有1株例外，因为它具有抗原O4。这43株中有33株具有K抗原K1。46株第2组菌株（AC50为0.001 - 0.009）的血清群分布为O2、O4、O6、O18ac、O75和O78；46株中有20株具有抗原K5。菌株的H抗原或血凝能力与AC50之间不存在相关性。一方面，AC50模式与两个主要致病组（即“新生儿脑膜炎”和“败血症及器官嗜性疾病”）之间，另一方面，AC50模式与O、K血清型之间显示出密切相关性。这些发现表明，具有相同标记的这些大肠杆菌菌株存在克隆联系。
