Bidet Philippe, Metais Arnaud, Mahjoub-Messai Farah, Durand Lionel, Dehem Marie, Aujard Yannick, Bingen Edouard, Nassif Xavier, Bonacorsi Stéphane
Service de Microbiologie, Hôpital Robert Debré, 48 Bd Sérurier, 75395 Paris cedex 19, France.
Appl Environ Microbiol. 2007 Apr;73(7):2373-7. doi: 10.1128/AEM.02341-06. Epub 2007 Feb 9.
Closely related Escherichia coli B2 strains O1:K1, O2:K1, O18:K1, and O45:K1 constitute a major subgroup causing extraintestinal infections. A DNA pathoarray analysis was used to develop a PCR specific for this subgroup that was included in the multiplex phylogenetic-grouping PCR method. Our PCR may serve to identify this virulent subgroup among different ecological niches.
密切相关的大肠杆菌B2菌株O1:K1、O2:K1、O18:K1和O45:K1构成了引起肠外感染的主要亚组。采用DNA病理芯片分析技术开发了一种针对该亚组的特异性PCR,并将其纳入多重系统发育分组PCR方法中。我们的PCR可用于在不同生态位中鉴定这一有毒力的亚组。