Peckham W D, Knobil E
Endocrinology. 1976 Apr;98(4):1054-60. doi: 10.1210/endo-98-4-1054.
A comparison of pituitary extracts from intact and ovariectomized monkeys revealed that, like FSH, LH from ovariectomized animals is larger in average apparent molecular size and disappears from the circulation of test rats less rapidly than does LH from intact females. In contrast, ovariectomy had no effect on the apparent molecular size of TSH nor on its disappearance rate following injection into test rats. Reversal of the effect of ovariectomy on the molecular size of both LH and FSH was achieved by the chronic administration of estradiol. The differences in apparent molecular size of gonadotropins from intact and ovariectomized monkeys were either reduced or abolished by digestion with the enzyme neuraminidase. These findings suggest that the qualitative changes in the gonadotropins consequent to ovariectomy represent a specific response to the removal of estrogen and are consonant with the view that this phenomenon involves changes in the sialic acid content of the gonadotropins.
对完整猴子和去卵巢猴子的垂体提取物进行比较发现，与促卵泡激素（FSH）一样，去卵巢动物的促黄体生成素（LH）平均表观分子大小更大，且从试验大鼠循环中消失的速度比完整雌性动物的LH慢。相比之下，去卵巢对促甲状腺激素（TSH）的表观分子大小及其注射到试验大鼠后的消失速率均无影响。通过长期给予雌二醇，可逆转去卵巢对LH和FSH分子大小的影响。用神经氨酸酶消化后，完整猴子和去卵巢猴子的促性腺激素表观分子大小差异要么减小，要么消失。这些发现表明，去卵巢后促性腺激素的质性变化是对雌激素去除的一种特异性反应，这与该现象涉及促性腺激素唾液酸含量变化的观点一致。
