Ammouche A, Dinh L, Youyou A, Clément M, Bourre J M
Institut national de la santé et de la recherche médicale, Unité 26, Hôpital Fernand Widal, France.
Biochem Cell Biol. 1993 Nov-Dec;71(11-12):530-7. doi: 10.1139/o93-076.
Studies were carried out to evaluate and relate the rate of alteration in mixed-function oxidase system with the changes of the fatty acid composition of rat microsomes induced by different dietary lipids. Male weanling rats were fed from day 21 to 120 with a commercial rat diet or a semisynthetic diet containing no fat or 10% fat consisting of peanut-rapeseed oil, sunflower oil, or salmon oil. In rats fed a fat-free diet, the cytochrome P-450 concentration and aniline hydroxylase, aminopyrine N-demethylase, and NADPH-cytochrome-c reductase activities of liver microsomes at 120 days were, respectively, 26, 16, 10, and 24% lesser than those of rats fed the control diet. However, cytochrome b5 concentration and NADH-cytochrome-b5 reductase activity were, respectively, 33 and 43% higher than those of the control group at the same time. When rats were fed the sunflower oil diet, the cytochrome P-450 concentration and NADH-cytochrome-b5 reductase activity at 120 days were, respectively, 11 and 23% lesser than those of control group. But the cytochrome b5 concentration was 10% higher than that of the control group. In rats fed the fish oil diet, the cytochrome P-450 concentration and NADPH-cytochrome-c reductase, aniline hydroxylase, and aminopyrine N-demethylase activities at 120 days were, respectively, 30, 48, 41, and 31% higher than those of rats fed the control diet. These enzymes were correlated very well (0.84 < r < 0.93), P < 0.05 with dietary sigma polyunsaturated fatty acids (n-3).(ABSTRACT TRUNCATED AT 250 WORDS)
开展了多项研究，以评估混合功能氧化酶系统的变化速率，并将其与不同膳食脂质诱导的大鼠微粒体脂肪酸组成变化相关联。从第21天到120天，给雄性断奶大鼠喂食市售大鼠饲料或不含脂肪或含10%脂肪（由花生油-菜籽油、向日葵油或鲑鱼油组成）的半合成饲料。在喂食无脂肪饲料的大鼠中，120天时肝脏微粒体的细胞色素P-450浓度以及苯胺羟化酶、氨基比林N-脱甲基酶和NADPH-细胞色素c还原酶活性分别比喂食对照饲料的大鼠低26%、16%、10%和24%。然而，细胞色素b5浓度和NADH-细胞色素b5还原酶活性在同一时间分别比对照组高33%和43%。当给大鼠喂食向日葵油饲料时，120天时细胞色素P-450浓度和NADH-细胞色素b5还原酶活性分别比对照组低11%和23%。但细胞色素b5浓度比对照组高10%。在喂食鱼油饲料的大鼠中，120天时细胞色素P-450浓度以及NADPH-细胞色素c还原酶、苯胺羟化酶和氨基比林N-脱甲基酶活性分别比喂食对照饲料的大鼠高30%、48%、41%和31%。这些酶与膳食中总多不饱和脂肪酸（n-3）的相关性非常好（0.84 < r < 0.93），P < 0.05。（摘要截断于250字）
