Imayama S, Nakamura K, Takeuchi M, Hori Y, Takema Y, Sakaino Y, Imokawa G
Department of Dermatology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
J Dermatol Sci. 1994 Feb;7(1):32-8. doi: 10.1016/0923-1811(94)90019-1.
We used scanning electron microscopy combined with perfusion fixation, resin injection and a selective digestion procedure to determine the effects of ultraviolet-B (UVB) radiation on the three-dimensional architecture of elastic fibers of rat skin. Chronic irradiation with a suberythematous dose of UVB (3 times/week for 12 weeks) produced a tortuous deformation of the superficial elastic fibers in the skin of the rat sole which normally are linearly arranged. Using computer analysis, we evaluated 40 individuals elastic fibers every 3 weeks for 12 weeks following the irradiation. This procedure confirmed the increasing tortuosity of the fibers, which was related to a decline in the elastic property of the skin in situ. Fine elastic branches developed among the deformed fibers and eventually anastomosed to produce an irregular network in the superficial dermal connective tissue, which may correspond to the development of actinic elastosis, that is, the UV-related accumulation of elastic fiber material.
我们采用扫描电子显微镜结合灌注固定、树脂注射和选择性消化程序，以确定紫外线B（UVB）辐射对大鼠皮肤弹性纤维三维结构的影响。用亚红斑剂量的UVB进行慢性照射（每周3次，共12周），导致大鼠足底皮肤中通常呈线性排列的浅表弹性纤维发生扭曲变形。在照射后的12周内，我们每3周使用计算机分析评估40条单个弹性纤维。该程序证实了纤维的曲折度增加，这与皮肤原位弹性特性的下降有关。在变形的纤维之间形成了细小的弹性分支，最终相互吻合，在浅表真皮结缔组织中形成不规则网络，这可能与光化性弹性组织变性的发展相对应，即与紫外线相关的弹性纤维物质积累。
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