Denis L T, Poindexter A N, Ritter M B, Seager S W, Deter R L
Fertil Steril. 1976 Jun;27(6):723-9.
These investigations were concerned with the response of squirrel monkey sperm to freezing. A procedure involving preincubation at 37 degrees C, progressive cooling combined with a two-step dilution, and freezing on Dry Ice was applied to semen samples collected by electroejaculation from two animals. Sperm concentrations in these samples were 321.8 X 10(6)/ml (+/-204.1 SD) and 532.8 X 10(6) ml (+/-117.1 SD), respectively. Sperm motility prior to freezing was 68.1% (+/- 15.9 SD) AND 63.6% (+/-14.8 SD). Following freezing and thawing, motilities of 53.8% (+/-17.1 SD) and 51.8% (+/-22.9 SD) were observed. The freezing procedure increased sperm motility in approximately 30% of samples from both animals. Changes in the motile behavior of sperm were not observed after freezing. Serum testosterone measurements, the first made in the squirrel monkey, gave values similar to those obtained for other primates. A progressive decline in testosterone concentration, seen in both animals during the course of these investigations, did not affect semen characteristics or the response of sperm to freezing.
这些研究关注松鼠猴精子对冷冻的反应。将一种涉及在37摄氏度下预孵育、逐步冷却并结合两步稀释以及在干冰上冷冻的程序应用于通过电刺激从两只动物采集的精液样本。这些样本中的精子浓度分别为321.8×10⁶/ml（±204.1标准差）和532.8×10⁶/ml（±117.1标准差）。冷冻前的精子活力分别为68.1%（±15.9标准差）和63.6%（±14.8标准差）。冷冻和解冻后，观察到的活力分别为53.8%（±17.1标准差）和51.8%（±22.9标准差）。冷冻程序使两只动物约30%的样本中的精子活力增加。冷冻后未观察到精子运动行为的变化。首次在松鼠猴身上进行的血清睾酮测量结果与在其他灵长类动物身上获得的结果相似。在这些研究过程中，两只动物体内睾酮浓度均呈逐渐下降趋势，但这并未影响精液特征或精子对冷冻的反应。
