Platenburg G J, Kootwijk E P, Kooiman P M, Woloshuk S L, Nuijens J H, Krimpenfort P J, Pieper F R, de Boer H A, Strijker R
GenePharming Europe B.V. Niels Bohrweg, Leiden, The Netherlands.
Transgenic Res. 1994 Mar;3(2):99-108. doi: 10.1007/BF01974087.
The expression of human lactoferrin (hLF) in the milk of transgenic mice is described. Regulatory sequences derived from the bovine alpha S1-casein gene were fused to the coding sequence of the hLF cDNA and several lines of transgenic mice were generated. Human LF RNA was detected exclusively in the mammary gland of lactating females and only after the onset of lactation. No aberrant RNA products could be detected using northern blotting and primer extension analysis. The hLF concentrations in the milk ranged from less than 0.1 to 36 micrograms ml-1. Human LF thus expressed did not differ from human milk derived LF, with respect to molecular mass and immunoreactivity with monoclonal and polyclonal antibodies.
本文描述了人乳铁蛋白（hLF）在转基因小鼠乳汁中的表达情况。将源自牛αS1-酪蛋白基因的调控序列与hLF cDNA的编码序列融合，并产生了数系转基因小鼠。仅在泌乳雌性小鼠的乳腺中检测到了人乳铁蛋白RNA，且是在泌乳开始后才检测到。通过Northern印迹法和引物延伸分析未检测到异常RNA产物。乳汁中的hLF浓度范围为每毫升低于0.1微克至36微克。如此表达的人乳铁蛋白在分子量以及与单克隆和多克隆抗体的免疫反应性方面与源自人乳的乳铁蛋白并无差异。
