Hoffner L, Deka R, Chakravarti A, Surti U
Department of Pathology, Magee-Womens Hospital, Pittsburgh, PA 15213.
Cancer Genet Cytogenet. 1994 May;74(1):54-8. doi: 10.1016/0165-4608(94)90029-9.
We present cytogenetic and DNA fingerprint analysis on 13 new cases of pediatric germ cell tumors; we analyze further four cases we have reported previously. The patients ranged in age from 23 weeks gestation to 16 years. The tumors were located in the ovary, sacrococcygeum, testis, mediastinum, and the craniofacial region, and represented benign, immature, and malignant cases. All of the new cases had a normal diploid karyotype. We have previously reported on multiple genetic mechanisms of origin for ovarian germ cell tumors, namely meiosis I nondisjunction, meiosis II nondisjunction, endoreduplication of a haploid ovum, mitotic division of premeiotic germ cell, and fusion of two ova. To determine the origin of extragonadal and testicular GCTs, Q-band centromeric heteromorphisms and DNA markers were analyzed in the host and the cognate tumor. Our data suggest that extragonadal and testicular GCTs do not arise by a meiosis I or II error, or by endoreduplication; rather, they arise mitotically from either a somatic or a germ cell.
我们对13例新发小儿生殖细胞肿瘤病例进行了细胞遗传学和DNA指纹分析，并进一步分析了我们之前报告过的4例病例。患者年龄从妊娠23周直至16岁。肿瘤位于卵巢、骶尾部、睾丸、纵隔及颅面部区域，包括良性、未成熟和恶性病例。所有新发的病例均具有正常的二倍体核型。我们之前曾报道过卵巢生殖细胞肿瘤的多种起源遗传机制，即减数分裂I不分离、减数分裂II不分离、单倍体卵子的核内复制、减数分裂前生殖细胞的有丝分裂以及两个卵子的融合。为了确定性腺外和睾丸生殖细胞肿瘤的起源，我们对宿主和相关肿瘤进行了Q带着丝粒异态性和DNA标记分析。我们的数据表明，性腺外和睾丸生殖细胞肿瘤并非由减数分裂I或II错误或核内复制产生；相反，它们是由体细胞或生殖细胞有丝分裂产生的。
