Hoffner L, Shen-Schwarz S, Deka R, Chakravarti A, Surti U
Department of Pathology, Magee-Womens Hospital, Pittsburgh, PA 15213.
Cancer Genet Cytogenet. 1992 Aug;62(1):58-65. doi: 10.1016/0165-4608(92)90040-f.
This report presents cytogenetic data on three cases of malignant ovarian germ cell tumors. All were diagnosed as malignant teratoma; case 1 with yolk sac elements; case 2 with elements of endodermal sinus tumor, embryonal carcinoma, and choriocarcinoma; and case 3 with yolk sac elements and embryonal carcinoma. Metaphase cells from each tumor, and normal tissue from the host, were karyotyped and scored for centromeric heteromorphisms in an attempt to determine the mechanism of origin. The karyotypes were 79,XXX,+1,+3,-6,+8,+12,+14,-15,+17, +20,+21,+22;49,XX,+8,+12,+22; and 48,XX,+3,+14, respectively. The analysis of centromeric heteromorphisms and DNA fingerprints of host and teratoma using the M13 probe revealed that one case originated from a germ cell before the first meiotic division. Normal host tissue was not available in case 2, but several centromeric markers were heterozygous in the tumor, indicating either meiosis I error or complete failure of germ cell meiosis. In the third case the centromeric heteromorphisms that were heterozygous in the host appeared to be homozygous for certain chromosomes and heterozygous for others in the tumor. These results suggest that germ cell teratomas could arise by the fusion of two ova.
本报告呈现了3例恶性卵巢生殖细胞肿瘤的细胞遗传学数据。所有病例均被诊断为恶性畸胎瘤；病例1含有卵黄囊成分；病例2含有内胚窦瘤、胚胎癌和绒毛膜癌成分；病例3含有卵黄囊成分和胚胎癌。对每个肿瘤的中期细胞以及宿主的正常组织进行核型分析，并对着丝粒异态性进行评分，以试图确定其起源机制。核型分别为79,XXX,+1,+3,-6,+8,+12,+14,-15,+17,+20,+21,+22；49,XX,+8,+12,+22；以及48,XX,+3,+14。使用M13探针分析宿主和畸胎瘤的着丝粒异态性和DNA指纹图谱显示，1例起源于第一次减数分裂前的生殖细胞。病例2没有宿主正常组织，但肿瘤中有几个着丝粒标记是杂合的，表明可能存在减数分裂I错误或生殖细胞减数分裂完全失败。在第3例中，宿主中杂合的着丝粒异态性在肿瘤中某些染色体上似乎是纯合的，而在其他染色体上是杂合的。这些结果表明，生殖细胞畸胎瘤可能由两个卵子融合产生。
