利用扫描质子微探针观察细胞内的抗艾滋病病毒药物。

The use of a scanning proton microprobe to observe anti-HIV drugs within cells.

作者信息

Cholewa M, Legge G J, Weigold H, Holan G, Birch C J

机构信息

Micro Analytical Research Centre (MARC), School of Physics, University of Melbourne, Parkville, Vic., Australia.

出版信息

Life Sci. 1994;54(21):1607-12. doi: 10.1016/0024-3205(94)90032-9.

DOI:10.1016/0024-3205(94)90032-9

PMID:8196479

Abstract

A series of inorganic polyanions (viz. heteropolytungstates) has been shown to have antiviral activity but there was no evidence to indicate that the drugs reached their site of antiviral (HIV) activity intact. We have shown that with a scanning proton microprobe it is possible to analyse the metal content of individual cells (PBLs) treated with such a polyoxometalate drug and to determine the atomic ratio of the metals within the cell. This was found to be near that in the drug. The distribution of the metals (tungsten and cobalt) within the cell was measured and it was shown that both metals were located in the same region within the cell. These findings would suggest that the drug had entered the cells intact.

摘要

一系列无机聚阴离子（即杂多钨酸盐）已被证明具有抗病毒活性，但没有证据表明这些药物能完整地到达其抗病毒（HIV）活性位点。我们已经表明，使用扫描质子微探针可以分析用这种多金属氧酸盐药物处理的单个细胞（外周血淋巴细胞）的金属含量，并确定细胞内金属的原子比。发现该比例与药物中的接近。测量了细胞内金属（钨和钴）的分布，结果表明两种金属都位于细胞内的同一区域。这些发现表明该药物已完整进入细胞。