Kamei J, Iwamoto Y, Hitosugi H, Misawa M, Nagase H, Kasuya Y
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan.
Life Sci. 1994;54(23):PL425-30. doi: 10.1016/0024-3205(94)90127-9.
The involvement of delta-opioid receptor subtypes in cold water swim stress (CWSS)-induced antinociception in diabetic mice was compared with that in non-diabetic mice. Three-minute swim stress produced significant antinociception in both diabetic and non-diabetic mice as determined by the tail-pinch test. However, the extent of CWSS-induced antinociception in diabetic mice was significantly greater than that in non-diabetic mice. Pretreatment with naltriben, a selective delta 2-opioid receptor antagonist, significantly attenuated CWSS-induced antinociception in both non-diabetic and diabetic mice. In contrast, although 7-benzylidenenaltrexone, a selective delta 1-opioid receptor antagonist, significantly attenuated CWSS-induced antinociception in diabetic mice, it had no effect in non-diabetic mice. These results suggest that CWSS-induced antinociception in non-diabetic mice is mediated by delta 2-opioid receptors, whereas CWSS-induced antinociception in diabetic mice is mediated by both delta 1- and delta 2-opioid receptors. Furthermore, the enhanced CWSS-induced antinociception in diabetic mice may be due to the activation of delta 1-opioid receptors.
将糖尿病小鼠与非糖尿病小鼠中δ-阿片受体亚型参与冷水游泳应激（CWSS）诱导的抗伤害感受作用进行了比较。通过夹尾试验确定，三分钟的游泳应激在糖尿病小鼠和非糖尿病小鼠中均产生了显著的抗伤害感受作用。然而，CWSS诱导的糖尿病小鼠抗伤害感受程度显著高于非糖尿病小鼠。用选择性δ2-阿片受体拮抗剂纳曲苄预处理，在非糖尿病和糖尿病小鼠中均显著减弱了CWSS诱导的抗伤害感受作用。相反，虽然选择性δ1-阿片受体拮抗剂7-苄叉基纳曲酮显著减弱了糖尿病小鼠中CWSS诱导的抗伤害感受作用，但在非糖尿病小鼠中却没有效果。这些结果表明，非糖尿病小鼠中CWSS诱导的抗伤害感受由δ2-阿片受体介导，而糖尿病小鼠中CWSS诱导的抗伤害感受由δ1-和δ2-阿片受体共同介导。此外，糖尿病小鼠中CWSS诱导的抗伤害感受增强可能是由于δ1-阿片受体的激活。
