Suh H W, Song D K, Kwon S H, Kim K W, Min B H, Kim Y H
Department of Pharmacology, College of Medicine, Hallym University, Chunchon, Korea.
Neuropeptides. 1996 Aug;30(4):379-84. doi: 10.1016/s0143-4179(96)90028-1.
We have previously reported that supraspinally and spinally located CCK receptors are involved in antagonizing supraspinally administered opioid-induced antinociception. Cold water swimming stress (CWSS) produces antinociception and opioid receptors are involved in CWSS-induced antinociception. The present study was designed to determine if supraspinal and spinal CCK receptors were involved in modulating the CWSS- induced antinociception. Antinociception was assessed by the tail-flick test. CWSS caused a profound inhibition of the tail-flick response. Various doses of CCK injected intracerebroventricularly (i.c.v.) or intrathecally (i.t.) alone did not show any antinociceptive effect. The i.c.v. or i.t. pretreatment with CCK (0.05-0.5 ng) dose dependently attenuated the CWSS- induced inhibition of the tail-flick response. In addition, i.c.v. pretreatment with lorglumide (0.1-10 pg) but not PD135,158 (1-100 pg) dose dependently reversed CCK's inhibition of the tail-flick response induced by CWSS. However, both lorglumide and PD135,158 injected i.t. reversed the antagonism of CCK against the inhibition of the tail-flick response induced by CWSS in a dose-dependent manner. Our results suggest that, at the supraspinal level, CCK(A) but not CCK(B) receptors may be involved in antagonizing the CWSS-induced antinociception. In the spinal cord both CCK(A) and CCK(B) receptors appear to be involved in antagonizing the CWSS-induced antinociception.
我们之前报道过，位于脊髓上和脊髓的胆囊收缩素（CCK）受体参与拮抗脊髓上给予阿片类药物诱导的镇痛作用。冷水游泳应激（CWSS）可产生镇痛作用，且阿片受体参与CWSS诱导的镇痛作用。本研究旨在确定脊髓上和脊髓的CCK受体是否参与调节CWSS诱导的镇痛作用。通过甩尾试验评估镇痛作用。CWSS对甩尾反应有显著抑制作用。单独脑室内注射（i.c.v.）或鞘内注射（i.t.）不同剂量的CCK均未显示出任何镇痛作用。脑室内或鞘内预先给予CCK（0.05 - 0.5 ng）剂量依赖性地减弱了CWSS诱导的甩尾反应抑制。此外，脑室内预先给予洛谷胺（0.1 - 10 pg）而非PD135,158（1 - 100 pg）剂量依赖性地逆转了CCK对CWSS诱导的甩尾反应的抑制。然而，鞘内注射洛谷胺和PD135,158均以剂量依赖性方式逆转了CCK对CWSS诱导的甩尾反应抑制的拮抗作用。我们的结果表明，在脊髓上水平，CCK（A）而非CCK（B）受体可能参与拮抗CWSS诱导的镇痛作用。在脊髓中，CCK（A）和CCK（B）受体似乎均参与拮抗CWSS诱导的镇痛作用。
