Giardino R, Faenza S, Spighi M, Fini M, Giavaresi G, Morrone G, Martini L
Department of Experimental Surgery, University of Bologna.
Boll Soc Ital Biol Sper. 1993 Oct;69(10):625-32.
We described some experimental models that were performed in rabbits and in swine in order to evaluate the efficacy of haemoperfusion treatment in hypochloremic alkalosis, uraemia and cytotoxic drug poisoning. In all the models, an extracorporeal circuit was used constituted mainly by a hematic sampling line and a cartridge, containing an anion exchange resin. Access to the blood stream was achieved by isolation and catheterization of the vessels either of the neck or of the leg, or both. The experimental model for the evaluation of haemoperfusion in hypochloremic alkalosis was carried out in rabbits by a pyloric stenosis because its size and weight are similar to new-born humans and its stomach is a simple monogastric one. The hypochloremia and alkalosis were achieved in only 4 hours. The other two experimental models were carried out in pigs because, in these cases, it was better to choose a large size animal with a nutritional similarity to humans, and with the capability to produce a stable chronic renal failure. The pigs were submitted to a bilateral ureter ligature to create a chronic renal failure or to a bilateral renal vessel ligature to avoid the physiologic precipitation of some drugs in renal tubules.
我们描述了一些在兔子和猪身上进行的实验模型，以评估血液灌流治疗在低氯性碱中毒、尿毒症和细胞毒性药物中毒方面的疗效。在所有模型中，均使用了一个体外循环装置，该装置主要由一条采血线和一个装有阴离子交换树脂的滤器组成。通过分离并插管颈部或腿部的血管，或同时插管这两处的血管来实现与血流的连通。评估低氯性碱中毒时血液灌流效果的实验模型是在兔子身上通过幽门狭窄来建立的，因为兔子的大小和体重与新生儿相似，且其胃是简单的单胃。仅4小时就实现了低氯血症和碱中毒。另外两个实验模型是在猪身上进行的，因为在这些情况下，最好选择一种体型较大、营养状况与人类相似且能够产生稳定慢性肾衰竭的动物。对猪进行双侧输尿管结扎以造成慢性肾衰竭，或进行双侧肾血管结扎以避免某些药物在肾小管中生理性沉淀。
