Davies M G, Hagen P O
Department of Surgery, Duke University Medical Center, Durham, NC 27710.
Ann Vasc Surg. 1994 Mar;8(2):150-7. doi: 10.1007/BF02018863.
It has been shown that suboptimal preparation of a vein graft prior to its insertion results in immediate morphologic and functional damage to the endothelial cells but not to the underlying smooth muscle cells. However, little is known about whether such perioperative injury to the vein grafts influences the subsequent development of intimal hyperplasia and smooth muscle cell contractility. This study examines the influence of storage in saline solution or Ringer's lactate on the development of intimal hyperplasia and vasomotor function in experimental vein grafts. Twenty-six New Zealand white rabbits had a carotid vein bypass graft performed after the veins had been immersed (15 minutes) in either heparinized saline solution (Sal; n = 13) or Ringer's lactate (RL; n = 13), and each group was harvested after 28 days for either histologic (n = 8) or functional studies (n = 5; four 5 mm rings/graft). Saline storage of the vein graft resulted in a 38% increase in the thickness of the intimal hyperplasia (113 +/- 2 vs. 83 +/- 2 microns, Sal vs. RL; mean +/- SEM; p < 0.05) without a change in medial thickness (87 +/- 5 vs. 86 +/- 8 microns, Sal vs. RL; p > 0.05). The two sets of vein grafts showed no difference in sensitivity to norepinephrine, serotonin, and bradykinin.(ABSTRACT TRUNCATED AT 250 WORDS)
研究表明，静脉移植物在植入前准备不充分会导致内皮细胞立即出现形态和功能损伤，但不会损伤其下方的平滑肌细胞。然而，关于静脉移植物的这种围手术期损伤是否会影响内膜增生和平滑肌细胞收缩性的后续发展，目前知之甚少。本研究考察了在盐溶液或乳酸林格液中保存对实验性静脉移植物内膜增生发展和血管舒缩功能的影响。26只新西兰白兔在静脉分别浸入肝素化盐溶液（盐溶液组；n = 13）或乳酸林格液（乳酸林格液组；n = 13）15分钟后进行颈动脉静脉搭桥移植，每组在28天后进行组织学研究（n = 8）或功能研究（n = 5；每个移植物取4个5毫米的环）。静脉移植物在盐溶液中保存导致内膜增生厚度增加38%（盐溶液组为113±2微米，乳酸林格液组为83±2微米；平均值±标准误；p < 0.05），而中膜厚度无变化（盐溶液组为87±5微米，乳酸林格液组为86±8微米；p > 0.05）。两组静脉移植物对去甲肾上腺素、5-羟色胺和缓激肽的敏感性无差异。（摘要截短于250词）
