具有t(4;11)染色体重排的急性淋巴细胞白血病细胞系SEM具有双表型，且对白介素-7有反应。

The acute lymphoblastic leukaemia cell line SEM with t(4;11) chromosomal rearrangement is biphenotypic and responsive to interleukin-7.

作者信息

Greil J, Gramatzki M, Burger R, Marschalek R, Peltner M, Trautmann U, Hansen-Hagge T E, Bartram C R, Fey G H, Stehr K

机构信息

Department of Paediatrics, University of Erlangen-Nuremberg, Germany.

出版信息

Br J Haematol. 1994 Feb;86(2):275-83. doi: 10.1111/j.1365-2141.1994.tb04726.x.

DOI:10.1111/j.1365-2141.1994.tb04726.x

PMID:8199015

Abstract

A cell line, designated SEM, was established from the peripheral blood of a 5-year-old girl in relapse with acute lymphoblastic leukaemia (ALL). Both the lymphoblasts of the patient and the cells of the cell line SEM showed the t(4;11) chromosomal rearrangement. The analysis of the immunophenotype of the SEM cell line revealed the B-cell differentiation antigens CD19, CD22 and CDw75 in the absence of CD20, CD24 and immunoglobulin expression. Besides B-lineage antigens, SEM cells were positive for the myeloid antigens CD13, CD15, CD33 and CDw65. Immunogenotypic analysis of SEM cells showed a monoclonal rearrangement of immunoglobulin heavy-chain (IgH). T-cell receptor (TCR) gamma and delta genes. Addition of interleukin (IL)-7 promoted the growth of the patient's lymphoblasts in culture and enhanced the proliferation of SEM cells. The SEM cells also express messenger RNA (mRNA) for the IL-7 receptor (IL-7R), but no evidence for autocrine production of IL-7 by the cell line was found. Addition of IL-4, tumour necrosis factor (TNF)-alpha, interferon (IFN)-alpha, or IFN-gamma resulted in a profound inhibition of SEM growth. Thus, these cytokines may have important growth regulatory activities for biphenotypic leukaemic ALL cells.

摘要

从一名复发急性淋巴细胞白血病（ALL）的5岁女孩外周血中建立了一个名为SEM的细胞系。患者的原始淋巴细胞和SEM细胞系的细胞均显示出t(4;11)染色体重排。对SEM细胞系免疫表型的分析显示存在B细胞分化抗原CD19、CD22和CDw75，而不存在CD20、CD24和免疫球蛋白表达。除了B系抗原外，SEM细胞的髓系抗原CD13、CD15、CD33和CDw65呈阳性。对SEM细胞的免疫基因型分析显示免疫球蛋白重链（IgH）、T细胞受体（TCR）γ和δ基因发生单克隆重排。添加白细胞介素（IL）-7可促进患者原始淋巴细胞在培养中的生长，并增强SEM细胞的增殖。SEM细胞还表达IL-7受体（IL-7R）的信使核糖核酸（mRNA），但未发现该细胞系自分泌IL-7的证据。添加IL-4、肿瘤坏死因子（TNF）-α、干扰素（IFN）-α或IFN-γ会导致SEM生长受到显著抑制。因此，这些细胞因子可能对双表型白血病ALL细胞具有重要的生长调节活性。