Konishi I, Ishikawa Y, Wang S Y, Wang D P, Koshiyama M, Mandai M, Komatsu T, Yamamoto S, Mori T
Department of Gynaecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.
Br J Obstet Gynaecol. 1994 Apr;101(4):325-9. doi: 10.1111/j.1471-0528.1994.tb13619.x.
To investigate the expression and distribution of transforming growth factor-alpha (TGF-alpha) in the normal cervix and in benign and malignant lesions of the uterine cervix.
Immuno-histochemical reactivity with a monoclonal antibody against TGF-alpha was examined in tissue specimens from 15 normal cervices, six cervical polyps, four cervical condylomata acuminata, 34 cervical intra-epithelial neoplasias, 35 invasive squamous cell carcinomas, five adenocarcinomas, and three mixed adenosquamous carcinomas.
Normal squamous cells of the exocervix were found to be negative for TGF-alpha immunoreactivity, whereas reserve cells and metaplastic squamous cells in the transformation zone were positive for TGF-alpha. Although TGF-alpha immuno-reactivity was variable in the cervical condylomas, most cases of cervical intra-epithelial neoplasia with or without koilocytotic atypia were negative for TGF-alpha. In the invasive carcinomas, however, TGF-alpha immuno-reactivity was observed in 17 out of the 35 cases of squamous carcinoma, and in all cases of adeno- and adenosquamous carcinomas. In addition, intense TGF-alpha immuno-reactivity was found in clinically advanced tumours.
These results suggest that the expression of TGF-alpha is associated with squamous metaplasia in the normal cervix, and that TGF-alpha may play an important role in cervical carcinogenesis, especially in its progression.
研究转化生长因子-α（TGF-α）在正常宫颈以及宫颈良性和恶性病变中的表达及分布情况。
采用抗TGF-α单克隆抗体对15例正常宫颈、6例宫颈息肉、4例宫颈尖锐湿疣、34例宫颈上皮内瘤变、35例浸润性鳞状细胞癌、5例腺癌及3例腺鳞癌的组织标本进行免疫组化反应检测。
发现宫颈外口的正常鳞状细胞TGF-α免疫反应呈阴性，而转化区的储备细胞和化生鳞状细胞TGF-α呈阳性。虽然宫颈湿疣中TGF-α免疫反应情况各异，但大多数伴或不伴挖空细胞异型性的宫颈上皮内瘤变病例TGF-α呈阴性。然而，在浸润癌中，35例鳞状细胞癌中有17例观察到TGF-α免疫反应，所有腺癌和腺鳞癌病例均呈阳性。此外，在临床晚期肿瘤中发现TGF-α免疫反应强烈。
这些结果表明，TGF-α的表达与正常宫颈的鳞状化生有关，并且TGF-α可能在宫颈癌发生过程中，尤其是在其进展过程中起重要作用。
