Ueyama Y, Misaki M, Ishihara Y, Matsumura T
Department of Oral and Maxillofacial Surgery II, Okayama University Dental School, Japan.
Br J Oral Maxillofac Surg. 1994 Apr;32(2):96-9. doi: 10.1016/0266-4356(94)90137-6.
The effects produced in vitro by minocycline (MINO), aminobenzyl penicillin (ABPC) and cefotiam (CTM) on polymorphonuclear leukocytes (PMN) chemotaxis were investigated in this study using the Boyden chamber technique and polarization assay. The following results were obtained: CTM brought about a significant increase of PMN chemotaxis at the therapeutic dose (10, 100 micrograms/ml), MINO reduced PMN chemotaxis significantly at a concentration as low as 1 microgram/ml and ABPC did not influence PMN chemotaxis at concentrations up to 100 micrograms/ml in both methods. Furthermore, using a checkerboard assay, it was shown that CTM worked as a chemokinetic factor.
本研究采用博伊登小室技术和极化分析，研究了米诺环素（MINO）、氨卞青霉素（ABPC）和头孢替安（CTM）在体外对多形核白细胞（PMN）趋化性的影响。获得了以下结果：在治疗剂量（10、100微克/毫升）下，CTM使PMN趋化性显著增加；在两种方法中，MINO在低至1微克/毫升的浓度下显著降低PMN趋化性，而ABPC在浓度高达100微克/毫升时不影响PMN趋化性。此外，使用棋盘分析表明，CTM作为一种化学促动因子起作用。
