O'Byrne E M, Steinetz B G
Proc Soc Exp Biol Med. 1976 Jun;152(2):272-6. doi: 10.3181/00379727-152-39377.
We have investigated the application of a RIA for porcine relaxin to the assay of relaxinlike substances in the blood of various other mammalian species. The cross-reactivity between antiporcine relaxin antibody and the relaxinlike substances in the blood of other mammals during pregnancy was sufficiently high to permit the assay of 0.1-0.5 ml of serum or plasma samples. Nonspecific reactivity was controlled by adding similar volumes of serum or plasma obtained from ovariectomized female or intact male subjects to RIA tubes containing known porcine standards. RIA relaxin levels rose markedly during the last third of pregnancy in rats, mice, and guinea pigs. RIA relaxin was also found in late pregnancy in dog, rhesus and java monkeys, and human beings. The apparent blood levels of hormone found in each species will depend upon their degree of cross-reactivity with the antiporcine relaxin antibody as well as upon their actual concentration. Thus, absolute blood level values should not be taken literally. However, the fluctuations in RIA relaxin observed during the course of gestation in any given species would appear to reflect accurately the relative blood concentrations of the hormone.
我们研究了猪松弛素放射免疫分析（RIA）在检测其他各种哺乳动物血液中类松弛素物质方面的应用。抗猪松弛素抗体与其他哺乳动物孕期血液中的类松弛素物质之间的交叉反应性足够高，足以检测0.1 - 0.5毫升血清或血浆样本。通过向含有已知猪标准品的RIA管中加入从卵巢切除的雌性或完整雄性个体获得的等量血清或血浆来控制非特异性反应。在大鼠、小鼠和豚鼠妊娠的最后三分之一期间，RIA检测的松弛素水平显著升高。在犬、恒河猴、爪哇猴和人类妊娠后期也发现了RIA检测的松弛素。在每个物种中发现的激素表观血液水平将取决于它们与抗猪松弛素抗体的交叉反应程度以及它们的实际浓度。因此，不应从字面上理解绝对血液水平值。然而，在任何给定物种的妊娠期观察到的RIA检测的松弛素波动似乎准确反映了该激素的相对血液浓度。
