Martiniello R, Smart Y C, Burton R C
Discipline of Surgical Science, Faculty of Medicine, University of Newcastle, N.S.W., Australia.
Cell Immunol. 1994 Jun;156(1):155-69. doi: 10.1006/cimm.1994.1161.
This study reports the generation of rat MoAb 2B6-F2 (IgG2a) that identifies a subpopulation of murine NK cells in the spleen, bone marrow, peripheral blood, lung, and peritoneal leukocytes. By flow cytometry, 2B6-F2 reacted with 10% of naive CBA (immunizing strain) spleen leukocytes of which 3% exhibited small lymphocyte morphology while 7% were large granular leukocytes. This pattern of binding was similarly obtained for BALB/c, C57BL/6, CE, and DBA/2 mice. 2B6-F2 and complement reduced splenic NK activity 40-57% in CBA, C57BL/6, BALB/c, CE, and DBA/2 strains. In CBA, C57BL/6, and CE mice < or = 15% reduction in splenic NC activity was observed while DBA/2 and BALB/c mice displayed 55-73% reduction. Cellular competitive inhibition studies showed that 2B6-F2+ NK cells in BALB/c and DBA/2 mice bind and kill YAC-1 and WEHI-164 target cells via a receptor that recognizes a shared determinant on these targets. Western blot and radioimmunoprecipitation studies indicated that 2B6-F2 identifies a 14-kDa monomeric cell surface molecule.
本研究报告了大鼠单克隆抗体2B6-F2（IgG2a）的产生，该抗体可识别脾脏、骨髓、外周血、肺和腹膜白细胞中的小鼠自然杀伤细胞亚群。通过流式细胞术，2B6-F2与10%的幼稚CBA（免疫原性菌株）脾脏白细胞发生反应，其中3%表现为小淋巴细胞形态，7%为大颗粒白细胞。对于BALB/c、C57BL/6、CE和DBA/2小鼠，也获得了类似的结合模式。在CBA、C57BL/6、BALB/c、CE和DBA/2品系中，2B6-F2与补体可使脾脏自然杀伤细胞活性降低40%-57%。在CBA、C57BL/6和CE小鼠中，脾脏自然细胞毒性活性降低≤15%，而DBA/2和BALB/c小鼠则降低55%-73%。细胞竞争性抑制研究表明，BALB/c和DBA/2小鼠中的2B6-F2+自然杀伤细胞通过识别这些靶标上共同决定簇的受体结合并杀伤YAC-1和WEHI-164靶细胞。蛋白质免疫印迹和放射免疫沉淀研究表明，2B6-F2识别一种14 kDa的单体细胞表面分子。
