Prieto M J, Castanho M, Coutinho A, Ortiz A, Aranda F J, Gómez-Fernández J C
Centro de Química Física Molecular, IST, Lisboa, Portugal.
Chem Phys Lipids. 1994 Jan;69(1):75-85. doi: 10.1016/0009-3084(94)90029-9.
A fluorescence study of a diacylglycerol derivatized with the n-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) chromophore (NBD) was carried out. Fluorescence self-quenching was observed for this probe in lecithin model membranes due to collisional interaction rather than to an aggregational behaviour of the probe. The efficient energy migration (Ro = 28 A) of the NBD fluorophore was studied via the fluorescence depolarization upon increase of probe concentration in membranes, and the results are compared with a model where a random distribution of the probes is assumed. A surface location of the chromophore was concluded for the NBD derivative of diacylglycerol, both from the fluorescence parameters and from the study of its fluorescence quenching by spin label probes. Very high lateral diffusion coefficients were obtained for these probes, both from the self-quenching (D = 2-6 x 10(-6) cm2 s-1) and from the spin probe quenching (D = 3.5 x 10(-6) cm2 s-1) studies. A concomitant fluorescence study of the related probe NBD-phosphatidylcholine revealed that its photophysical behaviour is similar to the derivatized diacylglycerol.
对用n-（7-硝基苯并-2-恶唑-1,3-二氮杂环丁烷-4-基）发色团（NBD）衍生化的二酰基甘油进行了荧光研究。由于碰撞相互作用而非探针的聚集行为，在卵磷脂模型膜中观察到该探针的荧光自猝灭现象。通过膜中探针浓度增加时的荧光去极化研究了NBD荧光团的有效能量迁移（Ro = 28 Å），并将结果与假设探针随机分布的模型进行了比较。从荧光参数以及自旋标记探针的荧光猝灭研究得出，二酰基甘油的NBD衍生物的发色团位于表面。从自猝灭（D = 2 - 6×10⁻⁶ cm² s⁻¹）和自旋探针猝灭（D = 3.5×10⁻⁶ cm² s⁻¹）研究中均获得了这些探针非常高的横向扩散系数。对相关探针NBD-磷脂酰胆碱的荧光研究表明，其光物理行为与衍生化的二酰基甘油相似。
