Loura L M, Fedorov A, Prieto M
Centro de Química-Física Molecular, Instituto Superior Técnico, Lisboa, Portugal.
Biophys J. 1996 Oct;71(4):1823-36. doi: 10.1016/S0006-3495(96)79383-9.
Resonance energy transfer between octadecyl rhodamine B (donor) and 1,1',3,3,3',3'-hexamethylindotricarbocyanine (acceptor) was studied in a model system of membranes (large unilamellar vesicles of dipalmitoylphosphatidylcholine), using both steady-state and time-resolved techniques. In the fluid phase (temperature = 50 degrees C) the decay law and the steady-state theoretical curve for energy transfer in two dimensions are verified. In the gel phase (temperature = 25 degrees C) an apparent reduction of dimensionality is observed, which is explained on the basis of probe segregation to the defect lines (grain boundaries). An estimation of the domain size from the model recovered linear probe concentrations is approximately 1750-2000 lipid molecules. In both phases, the existence of a fractal geometry was ruled out.
在膜（二棕榈酰磷脂酰胆碱的大单层囊泡）的模型系统中，使用稳态和时间分辨技术研究了十八烷基罗丹明B（供体）和1,1',3,3,3',3'-六甲基吲哚三碳菁（受体）之间的共振能量转移。在流体相（温度 = 50摄氏度）中，二维能量转移的衰减规律和稳态理论曲线得到了验证。在凝胶相（温度 = 25摄氏度）中，观察到维度明显降低，这是基于探针在缺陷线（晶界）处的偏析来解释的。根据从模型中恢复的线性探针浓度对域大小的估计约为1750 - 2000个脂质分子。在两个相中，均排除了分形几何结构的存在。
