Wang T, Diamandis E P, Lane A, Baines A D
Department of Clinical Biochemistry, Toronto General Division, Toronto Hospital, Ontario, Canada.
Clin Biochem. 1994 Feb;27(1):37-14. doi: 10.1016/0009-9120(94)90009-4.
Chloride measurements by ion-selective electrodes are vulnerable to interference by anions such as iodide, thiocyanate, nitrate, and bromide. We have found that the degree of interference of these anions on the Hitachi chemistry analyzer chloride electrode varies from electrode to electrode and this variation can even occur within the same lot of membrane. This variation is not dependent upon the length of time the cartridge has been in the analyzer because no correlation existed between the usage time and the electrode response to interfering ions. Neither is this variation due to the deterioration of the electrode because all electrodes tested had calibration slopes within the manufacturer's specification. Our study, however, showed that even after repeated exposure to a plasma sample containing 2 mM thiocyanate, the chloride electrode was still able to accurately measure the chloride in plasma without thiocyanate, thus confirming that a carryover effect does not exist from a previous thiocyanate-containing sample.
用离子选择电极测量氯化物容易受到碘化物、硫氰酸盐、硝酸盐和溴化物等阴离子的干扰。我们发现，这些阴离子对日立化学分析仪氯化物电极的干扰程度因电极而异，甚至在同一批膜内也会出现这种变化。这种变化并不取决于试剂盒在分析仪中放置的时间长短，因为使用时间与电极对干扰离子的响应之间不存在相关性。这种变化也不是由于电极老化所致，因为所有测试电极的校准斜率都在制造商规定的范围内。然而，我们的研究表明，即使在反复接触含有2 mM硫氰酸盐的血浆样本后，氯化物电极仍能准确测量不含硫氰酸盐的血浆中的氯化物，从而证实不存在来自先前含硫氰酸盐样本的残留效应。
