Wang J D, Shi W L, Zhang G Q, Bai X M
Department of Cell Biology, National Research Institute for Family Planning, Beijing, People's Republic of China.
Contraception. 1994 Mar;49(3):245-53. doi: 10.1016/0010-7824(94)90042-6.
The present study was designed to examine the effect RU 486 administration on steroid hormone levels in serum and decidua. Sixty women at 6-7 weeks gestation were divided into three groups. The first group took a placebo 24 hours before interruption of pregnancy. The other two groups took 200 mg mifepristone 12 and 24 hours before the surgical procedure, respectively. The concentrations of steroids and mifepristone were measured by radioimmunoassay or high performance liquid chromatography. Mifepristone treatment increased the levels of estradiol, cortisol and testosterone in serum and decidual cytosol (p < 0.05 or p < 0.01). A minor elevation in progesterone level was observed but was not statistically significant. The tissue levels of progesterone, estradiol and testosterone were much higher than the serum levels, whereas RU 486 concentration in the tissue was only one-third of the serum level. In addition, the RU 486 level in decidual cytosol was of the same order as that of progesterone. This competitive concentration was not achieved in chorionic villi in our previous observation, explaining why mifepristone exerts its predominant effect on decidua rather than villi. It is concluded that RU 486 reached an effective inhibitory concentration in decidua and had significant effects on the endocrine milieu.
本研究旨在探讨服用RU 486对血清及蜕膜中甾体激素水平的影响。60名妊娠6 - 7周的妇女被分为三组。第一组在终止妊娠前24小时服用安慰剂。另外两组分别在手术前12小时和24小时服用200毫克米非司酮。采用放射免疫分析法或高效液相色谱法测定甾体激素和米非司酮的浓度。米非司酮治疗可提高血清及蜕膜细胞溶质中雌二醇、皮质醇和睾酮的水平（p < 0.05或p < 0.01）。观察到孕酮水平有轻微升高，但无统计学意义。孕酮、雌二醇和睾酮的组织水平远高于血清水平，而组织中RU 486的浓度仅为血清水平的三分之一。此外，蜕膜细胞溶质中的RU 486水平与孕酮处于同一数量级。在我们之前的观察中，绒毛膜绒毛中未达到这种竞争浓度，这解释了为什么米非司酮对蜕膜而非绒毛发挥其主要作用。结论是，RU 486在蜕膜中达到有效抑制浓度，并对内分泌环境有显著影响。
