Shi W L, Wang J D, Fu Y, Xu L K, Zhu P D
Department of Cell Biology, National Research Institute for Family Planning, Beijing, People's Republic of China.
Hum Reprod. 1993 Jun;8(6):953-8. doi: 10.1093/oxfordjournals.humrep.a138173.
A total of 60 women with 6-7 weeks' amenorrhoea were randomly allocated to three groups. The women in the first group (control) took a placebo 24 h before undergoing vacuum aspiration. The subjects in the second and third groups were given orally 200 mg of RU 486, 12 or 24 h before surgical interruption of their pregnancy. Villi and decidua were collected and frozen in liquid nitrogen. There were no significant differences in villous cytosolic steroid concentrations between the control and RU 486-treated groups. The RU 486 concentration was lower in villous cytosol than in decidua and serum. The immunostaining of progesterone receptor in villous and extravillous trophoblast was weak and localized in both cytoplasm and nucleus. RU 486 treatment had no effect on the immunostaining of progesterone receptors in trophoblast populations except in the placental bed giant cells, where the weak and diffuse progesterone receptor staining in the cytoplasm of controls seemed to be concentrated in the nucleus after RU 486 treatment. In conclusion, RU 486 might have no effect on the immunostaining of progesterone receptor and on steroid concentrations in villi in vivo.
总共60名停经6 - 7周的女性被随机分为三组。第一组（对照组）女性在进行负压吸宫术前24小时服用安慰剂。第二组和第三组的受试者在手术终止妊娠前12或24小时口服200毫克米非司酮。收集绒毛和蜕膜并液氮冷冻。对照组和米非司酮治疗组之间绒毛细胞溶质类固醇浓度无显著差异。绒毛细胞溶质中的米非司酮浓度低于蜕膜和血清中的浓度。绒毛和绒毛外滋养层中孕激素受体的免疫染色较弱，定位于细胞质和细胞核。米非司酮治疗对滋养层细胞群体中孕激素受体的免疫染色没有影响，但在胎盘床巨细胞中，对照组细胞质中微弱且弥散的孕激素受体染色在米非司酮治疗后似乎集中到了细胞核中。总之，米非司酮在体内可能对孕激素受体的免疫染色和绒毛中的类固醇浓度没有影响。
