Shi W L, Wang J D, Xu L K, Fu Y, Zhu P D, Qiao G M, Wang Y Q
Department of Cell Biology, National Research Institute for Family Planning, Beijing, People's Republic of China.
Hum Reprod. 1992 Aug;7(7):934-9. doi: 10.1093/oxfordjournals.humrep.a137774.
Sixty patients with 6-7 weeks of amenorrhoea were randomly allocated to three groups. The women in the first group (control) took a placebo 24 h before undergoing a vacuum aspiration. The patients in the second and third groups were given 200 mg of RU 486 orally, 12 or 24 h before surgical interruption of their pregnancy. Decidua were collected and frozen in liquid nitrogen. By Scatchard plot analysis, the number of cytosolic binding sites (1798 +/- 803 fmol/mg DNA) of progesterone in decidua in the control group was significantly reduced (P less than 0.01) to 696 +/- 408 or 626 +/- 179 fmol/mg DNA by RU 486 treatment for 12 or 24 h respectively. The dissociation constants of both cytosolic and nuclear progesterone receptors in RU 486-exposed decidua were increased (P less than 0.01). The number of nuclear binding sites of oestrogen receptor was significantly higher (P less than 0.05) in decidua with RU 486 treatment for 12 h (178 +/- 77 fmol/mg DNA) compared to the control (89 +/- 32 fmol/mg DNA). The results suggest that RU 486 might regulate progesterone and oestrogen receptors in the decidua of early human pregnancy, either directly or indirectly.
60例闭经6 - 7周的患者被随机分为三组。第一组（对照组）的女性在进行负压吸引前24小时服用安慰剂。第二组和第三组的患者在手术终止妊娠前12小时或24小时口服200毫克RU 486。收集蜕膜并在液氮中冷冻。通过Scatchard作图分析，对照组蜕膜中孕酮的胞质结合位点数量（1798±803 fmol/mg DNA）在分别用RU 486处理12小时或24小时后显著降低（P＜0.01）至696±408或626±179 fmol/mg DNA。RU 486处理后的蜕膜中，胞质和核孕酮受体的解离常数均增加（P＜0.01）。与对照组（89±32 fmol/mg DNA）相比，用RU 486处理12小时的蜕膜中雌激素受体的核结合位点数量显著更高（P＜0.05）（178±77 fmol/mg DNA）。结果表明，RU 486可能直接或间接地调节早期人类妊娠蜕膜中的孕酮和雌激素受体。
