Bakøy O E, Thorstensen K
Department of Clinical Chemistry, University Hospital, Trondheim, Norway.
Eur J Biochem. 1994 May 15;222(1):105-12. doi: 10.1111/j.1432-1033.1994.tb18847.x.
In an attempt to improve our understanding of the complex interplay between cell compartments and chemical species during cellular uptake of iron from transferrin, we designed a computer simulation program based on current models of receptor-mediated endocytosis and pinocytosis. The program calculates and visualizes, as a function of time, the changes in transferrin, apotransferrin, and iron concentrations occurring in all relevant cellular compartments during cellular iron acquisition from transferrin. Simulation of literature data showed that the program generates results that are in accordance with experimental data. Furthermore, from measurements of the uptake of [carboxyl-14C]dextran we could utilize the program to suggest rate constants characteristic for the pinocytic process in rat reticulocytes. Moreover, simulations indicate that the apparent difference in the iron uptake process observed between reticulocytes and hepatocytes may be explained by the contribution made by pinocytosis to the iron uptake process. Finally, the present program should have potential as an educational tool during introduction to the field of receptor-mediated endocytosis in general and to cellular iron metabolism in particular.
为了更好地理解细胞从转铁蛋白摄取铁的过程中细胞区室与化学物质之间复杂的相互作用，我们基于目前受体介导的内吞作用和平胞饮作用模型设计了一个计算机模拟程序。该程序可以根据时间计算并可视化在细胞从转铁蛋白摄取铁的过程中，所有相关细胞区室中转铁蛋白、脱铁转铁蛋白和铁浓度的变化。对文献数据的模拟表明，该程序生成的结果与实验数据一致。此外，通过测量[羧基-14C]葡聚糖的摄取量，我们可以利用该程序来推测大鼠网织红细胞胞饮过程的特征速率常数。而且，模拟结果表明，网织红细胞和肝细胞在铁摄取过程中观察到的明显差异，可能是由于胞饮作用对铁摄取过程的贡献所致。最后，本程序作为一种教育工具，在一般的受体介导的内吞作用领域尤其是细胞铁代谢领域的入门教学中应该具有潜力。
