Vellani T S, Griffiths A J, Glass N L
Department of Botany, University of British Columbia, Vancouver, Canada.
Genome. 1994 Apr;37(2):249-55. doi: 10.1139/g94-035.
The mating-type locus in the ascomycete Neurospora crassa functions as a vegetative-incompatibility locus during asexual growth such that A+a heterokaryons and A/a partial diploids are inhibited in their growth. In this study, we sought mutations that suppress mating-type associated vegetative incompatibility by using A/a partial diploids. Mutants were selected as spontaneous escapes from inhibited growth. Suppressors were identified by selecting escapes that retained the capacity to mate with both A and a strains. The escaped partial diploids were then outcrossed to remove the duplication. Seven suppressors were identified that segregated as single, recessive mutations. All seven of the suppressors were shown to be allelic to a previously obtained suppressor, tol, by segregation analyses and heterokaryon tests. This result indicates that all seven mutations are new alleles of tol and suggests that tol is a key regulatory locus in the expression of mating-type associated vegetative incompatibility in N. crassa.
子囊菌粗糙脉孢菌中的交配型基因座在无性生长过程中作为营养不亲和基因座发挥作用，使得A+a异核体和A/a部分二倍体的生长受到抑制。在本研究中，我们利用A/a部分二倍体寻找抑制与交配型相关的营养不亲和性的突变。突变体是从受抑制生长中自发逃逸的个体中筛选出来的。通过选择保留与A和a菌株交配能力的逃逸个体来鉴定抑制子。然后将逃逸的部分二倍体进行杂交以去除重复序列。鉴定出7个抑制子，它们作为单隐性突变进行分离。通过分离分析和异核体测试表明，所有7个抑制子与先前获得的抑制子tol等位。这一结果表明，所有7个突变都是tol的新等位基因，并表明tol是粗糙脉孢菌中与交配型相关的营养不亲和性表达的关键调控基因座。
