Glass N L, Vollmer S J, Staben C, Grotelueschen J, Metzenberg R L, Yanofsky C
Department of Physiological Chemistry, University of Wisconsin, Madison 53706.
Science. 1988 Jul 29;241(4865):570-3. doi: 10.1126/science.2840740.
The mating-type alleles A and a of Neurospora crassa control mating in the sexual cycle and function in establishing heterokaryon incompatibility in the vegetative cycle. The A and a alleles were cloned, and they were shown to encode both the sexual functions and vegetative incompatibility. The mating-type clones contain nonhomologous DNA segments that are flanked by common DNA sequences. Neurospora crassa and all heterothallic and pseudohomothallic Neurospora species contain a single copy of one mating-type sequence or the other within each haploid genome. The six known self-fertile homothallic isolates contain an A homolog, but only one species also contains a homologous sequences. Homothallism in these species is not due to mating-type switching, as it is in Saccharomyces cerevisiae.
粗糙脉孢菌的交配型等位基因A和a在有性生殖周期中控制交配，并在营养生长周期中建立异核体不相容性方面发挥作用。A和a等位基因被克隆出来，并且显示它们既编码有性功能又编码营养生长不相容性。交配型克隆包含由共同DNA序列侧翼的非同源DNA片段。粗糙脉孢菌以及所有异宗配合和假同宗配合的脉孢菌物种在每个单倍体基因组中都含有一个交配型序列的单拷贝。六个已知的自交可育同宗配合分离株含有一个A同源物，但只有一个物种也含有同源序列。这些物种中的同宗配合不是像酿酒酵母那样由于交配型转换。
