Howell E C, Newbury H J, Swennen R L, Withers L A, Ford-Lloyd B V
School of Biological Sciences, University of Birmingham, Edgbaston, U.K.
Genome. 1994 Apr;37(2):328-32. doi: 10.1139/g94-045.
Using the technique of random amplified polymorphic DNA (RAPD), we have identified 116 amplification products in Musa germplasm using nine primers. This has enabled us to identify RAPD markers that are specific to each of nine genotypes of Musa representing AA, AAA, AAB, ABB, and BB genomes. The pattern of variation observed following the application of multivariate analyses to the RAPDs banding data is very similar to the pattern of variation defined using morphological characters and used to assign Musa material into the different genome classes.
利用随机扩增多态性DNA(RAPD)技术,我们使用9种引物在香蕉种质中鉴定出了116个扩增产物。这使我们能够鉴定出特定于代表AA、AAA、AAB、ABB和BB基因组的9种香蕉基因型中每一种的RAPD标记。对RAPD条带数据进行多变量分析后观察到的变异模式,与使用形态特征定义并用于将香蕉材料归入不同基因组类别的变异模式非常相似。