Bhat K V, Jarret R L, Rana R S
National Bureau of Plant Genetic Resources, New Delhi, India.
Electrophoresis. 1995 Sep;16(9):1736-45. doi: 10.1002/elps.11501601287.
Polymerase chain reaction (PCR) amplification of genomic DNA from 57 Musa cultivars with 60 random 10-mer primers generated 605 polymorphic amplification products which were useful in unambiguous cultivar identifications. Unweighted pair-group method analysis of this data grouped the cultivars into specific clusters depending on their genomic similarities. The diploid ancestral species of cultivated banana and plantains, namely Musa acuminata sp malaccensis, an A genome donor and M. balbisiana, a B genome donor, were farthest apart from each other in the phenogram. The edible fruit yielding cultivars with the genomic constitutions AA, AAA, AB, AAB, ABB, and ABBB grouped in different clusters according to overall genetic homologies. The restriction fragment length polymorphisms (RFLPs) prevalent among the cultivars were studied by hybridization of 19 random genomic clones to blots of HindIII, EcoRI and MspI digests. Cluster analysis of these data on 107 polymorphic alleles resulted in a phenogram comparable to the one obtained with random amplified polymorphic DNA (RAPD) analysis. Two multilocus probes useful in distinguishing all the 57 cultivars analyzed were also identified. The A and B types of cytoplasms in the cultivars were further distinguished by hybridization of heterologous chloroplast DNA probes. Results showed that use of different kinds of molecular markers in gene banks is essential for characterization and classification of germplasm collections.
用60个随机的10聚体引物对57个香蕉品种的基因组DNA进行聚合酶链反应（PCR）扩增，产生了605个多态性扩增产物，这些产物可用于明确的品种鉴定。对这些数据进行非加权配对组法分析，根据品种的基因组相似性将它们分为特定的簇。栽培香蕉和大蕉的二倍体祖先物种，即A基因组供体尖叶蕉（Musa acuminata sp malaccensis）和B基因组供体野蕉（M. balbisiana），在系统发育图中彼此相距最远。具有基因组组成AA、AAA、AB、AAB、ABB和ABBB的可食用果实品种根据总体遗传同源性分组在不同的簇中。通过将19个随机基因组克隆与HindIII、EcoRI和MspI消化物的印迹杂交，研究了品种中普遍存在的限制性片段长度多态性（RFLP）。对这107个多态性等位基因的数据进行聚类分析，得到的系统发育图与用随机扩增多态性DNA（RAPD）分析得到的图相似。还鉴定了两种可用于区分所有57个分析品种的多位点探针。通过异源叶绿体DNA探针杂交进一步区分了品种中的A和B型细胞质。结果表明，在基因库中使用不同类型的分子标记对于种质资源的鉴定和分类至关重要。
