Ramesh G R, Gopinathan K P
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
Gene. 1994 May 27;143(1):95-100. doi: 10.1016/0378-1119(94)90611-4.
The structural proteins of mycobacteriophage I3 have been analysed by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE), radioiodination and immunoblotting. Based on their abundance the 34- and 70-kDa bands appeared to represent the major structural proteins. Successful cloning and expression of the 70-kDa protein-encoding gene of phage I3 in Escherichia coli and its complete nucleotide sequence determination have been accomplished. A second (partial) open reading frame following the stop codon for the 70-kDa protein was also identified within the cloned fragment. The deduced amino-acid sequence of the 70-kDa protein and the codon usage patterns indicated the preponderance of codons, as predicted from the high G+C content of the genomic DNA of phage I3.
分枝杆菌噬菌体I3的结构蛋白已通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）、放射性碘化和免疫印迹法进行了分析。根据其丰度，34 kDa和70 kDa条带似乎代表主要结构蛋白。已成功在大肠杆菌中克隆并表达了噬菌体I3的70 kDa蛋白编码基因，并完成了其完整核苷酸序列的测定。在克隆片段中还鉴定出了70 kDa蛋白终止密码子之后的第二个（部分）开放阅读框。70 kDa蛋白的推导氨基酸序列和密码子使用模式表明，正如从噬菌体I3基因组DNA的高G+C含量所预测的那样，密码子占优势。
