Hatfull G F, Sarkis G J
Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260.
Mol Microbiol. 1993 Feb;7(3):395-405. doi: 10.1111/j.1365-2958.1993.tb01131.x.
Genetic studies of Mycobacterium tuberculosis and other mycobacterial pathogens have suffered from the lack of a sophisticated genetic system. To address this issue we have developed a viral system through a detailed characterization of mycobacteriophage L5, a temperate phage that infects both fast- and slow-growing mycobacteria. We describe here the complete DNA sequence of the L5 genome and initial characterization of L5 virion structure and gene expression. In addition to providing a genetic 'tool-box' for the mycobacteria we find that L5 offers a new paradigm for dsDNA phages, being phenotypically temperate but employing genetic strategies for phage growth usually associated with lytic bacteriophages.
结核分枝杆菌和其他分枝杆菌病原体的遗传学研究因缺乏精密的遗传系统而受到阻碍。为了解决这一问题，我们通过对分枝杆菌噬菌体L5进行详细表征，开发了一种病毒系统。L5是一种温和噬菌体，可感染快速生长和缓慢生长的分枝杆菌。我们在此描述了L5基因组的完整DNA序列以及L5病毒体结构和基因表达的初步表征。除了为分枝杆菌提供一个遗传“工具箱”外，我们发现L5为双链DNA噬菌体提供了一种新的模式，它在表型上是温和的，但采用了通常与裂解性噬菌体相关的噬菌体生长遗传策略。
