Kovacs D P, Tekpetey F R, Armstrong D T
Department of Physiology, University of Western Ontario, Canada.
Immunol Cell Biol. 1994 Apr;72(2):129-35. doi: 10.1038/icb.1994.20.
Porcine seminal plasma (PSP) contains a potent mitogenic substance capable of causing proliferation and extensive agglutination in cultured porcine peripheral blood leucocytes (PBL). In order to determine the specific leucocyte cell population affected by this mitogen, lymphocyte, monocyte and polymorphonuclear (PMN)-enriched cell fractions were separated from PBL and treated with increasing concentrations of PSP (1-8%, v/v). For monocyte cell populations enriched through elutriation and adherence to plastic, cell proliferation in response to PSP treatment was consistently higher than PSP-treated unfractionated PBL. Monocyte-depleted PBL and lymphocyte populations enriched through elutriation demonstrated a decrease in proliferation compared to PSP-treated unfractionated PBL. PMN populations separated from PBL by discontinuous Percoll gradient centrifugation were unresponsive to PSP. Agglutination was observed for the unfractionated PBL and each enriched cell population. These results demonstrate that PSP contains a potent mitogen which induces proliferation in monocyte-enriched cell populations and may reflect the potential of PSP to act as an immune regulator in the uterine environment during early embryo development and implantation.
猪精液血浆（PSP）含有一种强效促有丝分裂物质，能够在培养的猪外周血白细胞（PBL）中引起增殖和广泛凝集。为了确定受这种有丝分裂原影响的特定白细胞群体，从PBL中分离出淋巴细胞、单核细胞和多形核（PMN）富集细胞组分，并用递增浓度的PSP（1-8%，v/v）进行处理。对于通过淘洗和贴壁于塑料而富集的单核细胞群体，对PSP处理的细胞增殖始终高于PSP处理的未分级PBL。与PSP处理的未分级PBL相比，通过淘洗富集的单核细胞耗竭的PBL和淋巴细胞群体的增殖有所降低。通过不连续Percoll梯度离心从PBL中分离的PMN群体对PSP无反应。在未分级的PBL和每个富集细胞群体中均观察到凝集现象。这些结果表明，PSP含有一种强效有丝分裂原，可诱导单核细胞富集细胞群体中的增殖，并可能反映PSP在早期胚胎发育和着床期间子宫环境中作为免疫调节剂的潜力。
