Eriksson O, Enell B
Vox Sang. 1976;31(1):13-24. doi: 10.1111/j.1423-0410.1976.tb02854.x.
By incorporating whole Rh(+) red cells in an agarose plate and adding a small amount of purified 125I anti-D immunoglobulin as a tracer to standards and samples, a radioelectroimmunoassay (REIA) of anti-D immunoglobulin was possible. The results by this method agree with those obtained by the direct method. The coefficient of variation (within plate precision) is less than 9% and the reproducibility is 12% (coefficient of variation for a plasma sample assayed on six occasions during 1 month). The International anti-D serum was compared with the WHO standard preparation 68/419 on five occasions and found to contain 11; 11; 10; 9 and 11 mug per ampoule.
通过将完整的Rh(+)红细胞掺入琼脂糖平板中，并向标准品和样品中加入少量纯化的¹²⁵I抗-D免疫球蛋白作为示踪剂，抗-D免疫球蛋白的放射免疫电泳测定（REIA）成为可能。该方法的结果与直接法所得结果一致。变异系数（板内精密度）小于9%，重现性为12%（一个血浆样品在1个月内进行6次测定的变异系数）。国际抗-D血清与世界卫生组织标准制剂68/419进行了5次比较，发现每安瓿含11；11；10；9和11微克。
