Romano E L, Stolinski C, Hughes-Jones N C
Br J Haematol. 1975 Aug;30(4):507-16. doi: 10.1111/j.1365-2141.1975.tb01865.x.
Studies are presented in which a gold-labelled anti-IgG reagent has been used to map the distribution of A, D and c antigen sites on the human red cell membrane. Red cells were combined with IgG antibodies; ghosts were produced which were then labelled with gold-anti-IgG. D antigen sites were found to be single entities dispersed on the red cell membrane of lysed but otherwise untreated Rh positive cells. Following papain treatment prior to lysis, a clustered distribution was observed. Clustering of D sites was also observed on non-enzyme-treated red cells of the -D- phenotype. Further studies indicated that D and c antigen sites are closely associated on ccDEE red cells. The distribution of A antigen sites was found to be clustered in proportion to the amount of IgG anti-A combined with the cells. Reasons are given for considering such distribution as indirect evidence of mobility of A sites. There was an association between agglutinability of red cells by IgG antibodies and the extent of the clustering of the antigen sites.
本文展示了多项研究，其中使用了金标记的抗IgG试剂来绘制人红细胞膜上A、D和c抗原位点的分布情况。红细胞与IgG抗体结合；产生了红细胞影，然后用金标抗IgG进行标记。发现D抗原位点是分散在已裂解但未作其他处理的Rh阳性细胞红细胞膜上的单个实体。在裂解前进行木瓜蛋白酶处理后，观察到呈簇状分布。在-D-表型的未酶处理红细胞上也观察到D位点的聚集。进一步研究表明，在ccDEE红细胞上，D和c抗原位点紧密相关。发现A抗原位点的分布与结合到细胞上的IgG抗A的量成比例地聚集。给出了将这种分布视为A位点可移动性间接证据的理由。红细胞被IgG抗体凝集的能力与抗原位点的聚集程度之间存在关联。
